| Full text | |
| Author(s): Show less - |
Amorim, Vanessa G.
[1]
;
Melo, Shaiani M. G.
[2]
;
Leite, Ruan F.
[3]
;
Coutinho, Paulo Arthur
[1]
;
da Silva, Sarah M. P.
[1, 4]
;
da Silva, Andre R.
[1, 5]
;
Amorim, Fernanda G.
[6]
;
Pires, Rita G. W.
[1, 4]
;
Coitinho, Juliana B.
[1]
;
Emery, Flavio S.
[2]
;
Rezende, Lucas C. D.
[1, 3]
Total Authors: 11
|
| Affiliation: | [1] Univ Fed Espirito Santo, Grad Program Biochem, Vitoria, ES - Brazil
[2] Univ Sao Paulo FCFRP USP, Sch Pharmaceut Sci Ribeirao Preto, Dept Pharmaceut Sci, Ribeirao Preto, SP - Brazil
[3] Univ Fed Espirito Santo, Dept Nat Sci, Sao Mateus, ES - Brazil
[4] Univ Fed Minas Gerais, Grad Program Biochem & Immunol, Belo Horizonte, MG - Brazil
[5] Fed Inst Espirito Santo, Campus Aracruz, Aracruz, ES - Brazil
[6] Vila Velha Univ, Vila Velha, ES - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | DYES AND PIGMENTS; v. 182, NOV 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Fluorescent organic small molecules are very important tools for researchers in the fields of biochemistry and biotechnology, and boron-dipyrromethene (BODIPY) class of organic fluorophores gained much attention in recent years due to their remarkable photophysical properties and chemical versatility. Their application often involves conjugation with biomolecules, which can be accomplished by inserting a reactive functionality to the fluorescent dye. In this paper we present the preparation and characterization of two unpublished BODIPYs bearing the electrophilic isothiocyanate group, widely used for conjugation with proteins and biological amines. These dyes were prepared by inserting a nitrobenzene group to the BODIPY core via direct arylation, which was later converted to the isothiocyanate functionality. The arylated BODIPYs retained their fluorescence and showed red-shifted absorption and fluorescence spectra, when compared to previously published isothiocyanateBODIPYs. Their application in bioconjugation was tested with bovine serum albumin (BSA) and was confirmed from spectroscopic and electrophoretic analysis. (AU) | |
| FAPESP's process: | 14/18973-8 - Application of diazonium salts chemistry in structural modification of BODIPY fluorophores |
| Grantee: | Shaiani Maria Gil de Melo |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 13/24408-9 - Chemical modification of BODIPY fluorophores aiming at structural and photophysical diversity for the development of novel fluorescent probes |
| Grantee: | Lucas Cunha Dias de Rezende |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 11/23342-9 - Synthesis, characterization and applications of BODIPY fluorophores. |
| Grantee: | Lucas Cunha Dias de Rezende |
| Support Opportunities: | Scholarships in Brazil - Doctorate |