| Full text | |
| Author(s): |
Amorim, Tatiane Lima
[1, 2]
;
Duarte, Lucas Mattos
[1, 3]
;
Granato, Alisson Silva
[4]
;
Oliveira, Marcone Augusto Leal de
[1]
;
Amarante, Giovanni Wilson
[4]
;
Fuente, Miguel Angel de la
[2]
;
Gomez-Cortes, Pilar
[2]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed Juiz de Fora, Grp Quim Analit & Quimiometria GQAQ, BR-36036900 Juiz De Fora, MG - Brazil
[2] Univ Autonoma Madrid, CSIC, Inst Invest Ciencias Alimentac CIAL, Nicolas Cabrera 9, Madrid 28049 - Spain
[3] Univ Fed Fluminense, Inst Quim, Dept Quim Analit, BR-24020141 Niteroi, RJ - Brazil
[4] Univ Fed Juiz De Fora, Grp Pesquisas Metodol Sintet GPMS, BR-36036900 Juiz De Fora, MG - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | LWT-FOOD SCIENCE AND TECHNOLOGY; v. 131, SEP 2020. |
| Web of Science Citations: | 1 |
| Abstract | |
The regulations established worldwide, restricting trans fatty acids (TFA) from industrial origin in foods, encourage the development of rapid methods to verify the quality of food products. A simple and rapid procedure based on H-1 NMR combined with multivariate calibration was developed to quantify C18:1 TFA isomers in chocolate fat. Thirty chocolate samples (milk, dark and white chocolates) were analyzed by H-1 NMR, and also by a reference gas chromatographic method. Then, partial least squares regression was used for model development. The most important H-1 NMR signals for the modeling comprised the 5.25-5.45 ppm region, corresponding to CH (double bonds and glycerol backbone), and the 1.94-1.99 ppm region, characteristic of CH2 allylic to trans double bonds. Good predictive qualities for all TFA isomers were obtained. Coefficient of correlation for prediction was 0.967 for C18:1 trans 6-8; 0.981 for C18:1 trans 9; 0.987 for C18:1 trans 10; 0.941 for C18:1 trans 11; 0.983 for C18:1 trans 12 and 0.977 for total TFA. This study demonstrates the potential of H-1 NMR to be applied in food industries in order to perform TFA control analysis as well as to investigate food fraud. (AU) | |
| FAPESP's process: | 14/50867-3 - Inct 2014 - de bioanalitica. |
| Grantee: | Marco Aurelio Zezzi Arruda |
| Support Opportunities: | Research Projects - Thematic Grants |