Interesterified palm oil impairs glucose homeostasis and induces deleterious effects in liver of Swiss mice

Author(s):Show less -

Miyamoto, Josiane Erica ^{[1, 2]} ; Reginato, Andressa ^{[1, 2]} ; Portovedo, Mariana ^{[1, 2]} ; dos Santos, Raisa Magno ^{[1, 2]} ; Stahl, Marcella Aparecida ^[3] ; Le Stunff, Herve ^[4] ; Latorraca, Marcia Queiroz ^[5] ; de Barros Reis, Marise Auxiliadora ^[5] ; Arantes, Vanessa Cristina ^[5] ; Doneda, Diego Luiz ^[6] ; Ignacio-Souza, Leticia Martins ^{[1, 2]} ; Torsoni, Adriana Souza ^{[1, 2]} ; Grimaldi, Renato ^[3] ; Badan Ribeiro, Ana Paula ^[3] ; Torsoni, Marcio Alberto ^{[1, 2]} ; Milanski, Marciane ^{[1, 2]}

Affiliation

^[1] Univ Estadual Campinas, UNICAMP, Sch Appl Sci, Limeira - Brazil

^[2] Univ Estadual Campinas, Obes & Comorbid Res Ctr, UNICAMP, Campinas - Brazil

^[3] Univ Estadual Campinas, Sch Food Engn, UNICAMP, Campinas - Brazil

^[4] Univ Paris Saclay, Paris Saclay Inst Neurosci, Univ Paris Sud, CNRS, UMR 9197, Orsay - France

^[5] Univ Fed Mato Grosso, UFMT, Nutr Fac, Cuiaba - Brazil

^[6] Univ Fed Mato Grosso, Dept Basic Hlth Sci, Physiol Lab, Cuiaba - Brazil

• Document type: Journal article
• Source: METABOLISM-CLINICAL AND EXPERIMENTAL; v. 112, NOV 2020.
• Web of Science Citations: 1

Abstract

Background: Interesterified fats have largely replaced the partially hydrogenated oils which are the main dietary source of trans fat in industrialized food. This process promotes a random rearrangement of the native fatty acids and the results are different triacylglycerol (TAG) molecules without generating trans isomers. The role of interesterified fats in metabolism remains unclear. We evaluated metabolic parameters, glucose homeostasis and inflammatory markers in mice fed with normocaloric and normolipidic diets or hypercaloric and high-fat diet enriched with interesterified palm oil. Methods: Male Swiss mice were randomly divided into four experimental groups and submitted to either normolipidic palm oil diet (PO), normolipidic interesterified palm oil diet (IPO), palm oil high-fat diet (POHF) or interesterified palm oil high-fat diet (IPOHF) during an 8 weeks period. Results: When compared to the PO group, IPO group presented higher body mass, hyperglycemia, impaired glucose tolerance, evidence of insulin resistance and greater production of glucose in basal state during pyruvate in situ assay. We also observed higher protein content of hepatic PEPCK and increased cytokine mRNA expression in the IPO group when compared to PO. Interestingly, IPO group showed similar parameters to POHF and IPOHE groups. Conclusion: The results indicate that substitution of palm oil for interesterified palm oil even on normocaloric and normolipidic diet could negatively modulate metabolic parameters and glucose homeostasis as well as cytokine gene expression in the liver and white adipose tissue. This data support concerns about the effects of interesterified fats on health and could promote further discussions about the safety of the utilization of this unnatural fat by food industry. (C) 2020 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 16/24768-3 - Evaluation of metabolic parameters of experimental animal models in response to dietary interesterified fat: underlaying molecular mechanisms
• Grantee: Josiane Érica Miyamoto
• Support Opportunities: Scholarships in Brazil - Doctorate