Strength exercise reduces hepatic pyruvate carboxylase and gluconeogenesis in DIO mice

Author(s):Show less -

Pereira, Rodrigo Martins ^[1] ; da Cruz Rodrigues, Kellen Cristina ^[1] ; Sant'Ana, Marcella Ramos ^[2] ; Peruca, Guilherme Francisco ^[1] ; Morelli, Ana Paula ^[3] ; Simabuco, Fernando M. ^[3] ; da Silva, Adelino S. R. ^{[4, 5]} ; Cintra, Dennys Esper ^[2] ; Ropelle, Eduardo Rochete ^[6] ; Pauli, Jose Rodrigo ^[6] ; de Moura, Leandro Pereira ^[1]

Affiliation

^[1] Univ Estadual Campinas, Fac Appl Sci, Exercise Cell Biol Lab, Limeira - Brazil

^[2] Univ Estadual Campinas, Fac Appl Sci, Lab Nutr Genom, Limeira - Brazil

^[3] Univ Estadual Campinas, Fac Appl Sci, Multidisciplinary Lab Food & Hlth, Limeira - Brazil

^[4] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Ribeirao Preto Med Sch, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto, SP - Brazil

^[6] State Univ Campinas UNICAMP, Fac Appl Sci, Lab Mol Biol Exercise LaBMEx, Limeira, SP - Brazil

• Document type: Journal article
• Source: Journal of Endocrinology; v. 247, n. 2, p. 127-138, NOV 2020.
• Web of Science Citations: 0

Abstract

Obesity is linked to a reduction in the control of hepatic glucose production, which is the primary mechanism related to fasting hyperglycemia and the development of type 2 diabetes mellitus (T2DM). The main system involved in hepatic gluconeogenesis synthesis is controlled by pyruvate carboxylase (PC), which increases in obesity conditions. Recently, we showed that short-term strength training is an important tool against obesity-induced hyperglycemia. As aerobic exercise can reduce the hepatic PC content of obese animals, we hypothesized that strength exercise can also decrease this gluconeogenic enzyme. Therefore, this study investigated whether the metabolic benefits promoted by short-term strength training are related to changes in hepatic PC content. Swiss mice were divided into three groups: lean control (Ctl), obese sedentary (ObS), and obese short-term strength training (STST). The STST protocol was performed through one session/day for 15 days. The obese exercised animals had reduced hyperglycemia and insulin resistance. These results were related to better control of hepatic glucose production and hepatic insulin sensitivity. Our bioinformatics analysis showed that hepatic PC mRNA levels have positive correlations with glucose levels and adiposity, and negative correlations with locomotor activity and muscle mass. We also found that hepatic mRNA levels are related to lipogenic markers in the liver. Finally, we observed that the obese animals had an increased hepatic PC level; however, STST was efficient in reducing its amount. In conclusion, we provide insights into new biomolecular mechanisms by showing how STST is an efficient tool against obesityrelated hyperglycemia and T2DM, even without body weight changes. (AU)

• FAPESP's process: 16/12569-6 - Effects of different exercise intensities on clusterin metabolism and its interference on insulin signaling in rodents
• Grantee: Rodrigo Martins Pereira
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 15/07199-2 - Role of clusterin/ApoJ on insulin signalling in response to physical exercise in rodents and humans
• Grantee: Leandro Pereira de Moura
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 16/24406-4 - The role of physical exercise in pathways signaling of clusterin/leptin in hypothalamus of obese mice
• Grantee: Kellen Cristina da Cruz Rodrigues
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)