Valerino-Diaz, Alexander B.
Zanatta, Ana C.
Bolela Bovo Candido, Ana Carolina
Magalhaes, Lizandra Guidi
dos Santos, Lourdes Campaner
Total Authors: 7
 Sao Paulo State Univ, UNESP, Inst Chem, Rua Prof Francisco Degni 55, BR-14800060 Araraquara, SP - Brazil
 Univ Franca, Ctr Res Sci & Technol, Res Grp Nat Prod, Av Dr Armando Salles Oliveira 201, BR-14404600 Franca, SP - Brazil
 Sao Paulo State Univ, UNESP, Inst Biosci, Praia Infante Dom Henrique S-N, BR-11330900 Sao Vicente, SP - Brazil
Total Affiliations: 3
Journal of Pharmaceutical and Biomedical Analysis;
NOV 30 2020.
Web of Science Citations:
Solanum paniculatum L. is species whose fruits are widely consumed in Brazil as a tonic beverage with higher content of steroidal saponins. In this work, we developed an analytical method for the quantification of the eight saponins present in the 70 % ethanol extract from the leaves using ultra-high-performance liquid chromatography coupled to mass spectrometry (UHPLC-MS). Besides, the eight spirostanic saponins were screened for in vitro antileishmanial activity against promastigote and amastigote forms of Leishmania (L.) amazonensis. Substances 1, 2 and 3 were found to be the most active compounds, with inhibitory concentration (IC50) values of 8.51 +/- 4.38, 10.75 +/- 6.85 and 10.45 +/- 4.21 mu M, respectively, against promastigote forms and effective concentration (EC50) values of >25, 17.73 +/- 0.99 and 19.57 +/- 0.84 mu M, respectively, against amastigote forms. The cytotoxic test with compounds 1-3 evidenced low toxicity in murine macrophage cells, with values above 50 mu M at concentration lower than 25 mu M. These findings show that saponins 1-3 should be evaluated in further studies for the treatment of cutaneous leishmaniasis. (C) 2020 Elsevier B.V. All rights reserved. (AU)