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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

An enquiry into antileishmanial activity and quantitative analysis of polyhydroxylated steroidal saponins from Solanum paniculatum L. leaves

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Author(s):
Valerino-Diaz, Alexander B. [1] ; Zanatta, Ana C. [1] ; Gamiotea-Turro, Daylin [1] ; Bolela Bovo Candido, Ana Carolina [2] ; Magalhaes, Lizandra Guidi [2] ; Vilegas, Wagner [3] ; dos Santos, Lourdes Campaner [1]
Total Authors: 7
Affiliation:
[1] Sao Paulo State Univ, UNESP, Inst Chem, Rua Prof Francisco Degni 55, BR-14800060 Araraquara, SP - Brazil
[2] Univ Franca, Ctr Res Sci & Technol, Res Grp Nat Prod, Av Dr Armando Salles Oliveira 201, BR-14404600 Franca, SP - Brazil
[3] Sao Paulo State Univ, UNESP, Inst Biosci, Praia Infante Dom Henrique S-N, BR-11330900 Sao Vicente, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Pharmaceutical and Biomedical Analysis; v. 191, NOV 30 2020.
Web of Science Citations: 0
Abstract

Solanum paniculatum L. is species whose fruits are widely consumed in Brazil as a tonic beverage with higher content of steroidal saponins. In this work, we developed an analytical method for the quantification of the eight saponins present in the 70 % ethanol extract from the leaves using ultra-high-performance liquid chromatography coupled to mass spectrometry (UHPLC-MS). Besides, the eight spirostanic saponins were screened for in vitro antileishmanial activity against promastigote and amastigote forms of Leishmania (L.) amazonensis. Substances 1, 2 and 3 were found to be the most active compounds, with inhibitory concentration (IC50) values of 8.51 +/- 4.38, 10.75 +/- 6.85 and 10.45 +/- 4.21 mu M, respectively, against promastigote forms and effective concentration (EC50) values of >25, 17.73 +/- 0.99 and 19.57 +/- 0.84 mu M, respectively, against amastigote forms. The cytotoxic test with compounds 1-3 evidenced low toxicity in murine macrophage cells, with values above 50 mu M at concentration lower than 25 mu M. These findings show that saponins 1-3 should be evaluated in further studies for the treatment of cutaneous leishmaniasis. (C) 2020 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 09/52237-9 - Standardized herbal medicines for the treatment of chronic diseases
Grantee:Wagner Vilegas
Support type: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 16/21044-4 - Standardization of phytomedicines for the treatment of chronic diseases
Grantee:Ana Caroline Zanatta Silva
Support type: Scholarships in Brazil - Doctorate