Neighbor danger: Yellow fever virus epizootics in urban and urban-rural transition areas of Minas Gerais state, during 2017-2018 yellow fever outbreaks in Brazil

Author(s):Show less -

Sacchetto, Livia ^[1] ; Oliveira Silva, Natalia Ingrid ^[1] ; de Rezende, Izabela Mauricio ^[1] ; Arruda, Matheus Soares ^[1] ; Costa, Thais Alkifeles ^[1] ; de Mello, Erica Munhoz ^[2] ; Garcia Oliveira, Gabriela Fernanda ^[1] ; Alves, Pedro Augusto ^[3] ; de Mendonca, Vitor Emidio ^[4] ; Antonelli Vidal Stumpp, Rodolfo German ^[5] ; Alves Prado, Alaine Izabela ^[5] ; Paglia, Adriano Pereira ^[5] ; Perini, Fernando Araujo ^[4] ; Nogueira, Mauricio Lacerda ^[6] ; Kroon, Erna Geessien ^[1] ; de Thoisy, Benoit ^[7] ; Trindade, Giliane de Souza ^[1] ; Drumond, Betania Paiva ^[1]

Affiliation

^[1] Univ Fed Minas Gerais, Dept Microbiol, Belo Horizonte, MG - Brazil

^[2] Ctr Controle Zoonoses, Belo Horizonte, MG - Brazil

^[3] Fundacao Oswaldo Cruz Minas, Inst Rene Rachou, Belo Horizonte, MG - Brazil

^[4] Univ Fed Minas Gerais, Dept Zool, Belo Horizonte, MG - Brazil

^[5] Univ Fed Minas Gerais, Dept Genet Ecol & Evolut, Belo Horizonte, MG - Brazil

^[6] Fac Med Sao Jose do Rio Preto, Dept Dermatol Infect & Parasit Dis, Sao Paulo - Brazil

^[7] Inst Pasteur, Cayenne - French Guiana

• Document type: Journal article
• Source: PLoS Neglected Tropical Diseases; v. 14, n. 10 OCT 2020.
• Web of Science Citations: 0

Abstract

Background From the end of 2016 until the beginning of 2019, Brazil faced a massive sylvatic yellow fever (YF) outbreak. The 2016-2019 YF epidemics affected densely populated areas, especially the Southeast region, causing thousands of deaths of humans and non-human primates (NHP). Methodology/Principal findings We conducted a molecular investigation of yellow fever virus (YFV) RNA in 781 NHP carcasses collected in the urban, urban-rural interface, and rural areas of Minas Gerais state, from January 2017 to December 2018. Samples were analyzed according to the period of sampling, NHP genera, sampling areas, and sampling areas/NHP genera to compare the proportions of YFV-positive carcasses and the estimated YFV genomic loads. YFV infection was confirmed in 38.1% of NHP carcasses (including specimens of the generaAlouatta,Callicebus,Callithrix, andSapajus), from the urban, urban-rural interface, and rural areas. YFV RNA detection was positively associated with epidemic periods (especially from December to March) and the rural environment. Higher median viral genomic loads (one million times) were estimated in carcasses collected in rural areas compared to urban ones. Conclusions/Significance The results showed the wide occurrence of YF in Minas Gerais in epidemic and non-epidemic periods. According to the sylvatic pattern of YF, a gradient of viral dissemination from rural towards urban areas was observed. A high YF positivity was observed for NHP carcasses collected in urban areas with a widespread occurrence in 67 municipalities of Minas Gerais, including large urban centers. Although there was no documented case of urban/AedesYFV transmission to humans in Brazil during the 2016-2019 outbreaks, YFV-infected NHP in urban areas with high infestation byAedes aegyptiposes risks for YFV urban/Aedestransmission and urbanization. Author summary Brazil faced the most massive sylvatic yellow fever (YF) outbreak in 2016-2019. The outbreak affected highly densely populated areas, and Minas Gerais was the most affected state with thousands of deaths of human and non-human primates (NHP). We investigated the yellow fever virus (YFV) RNA in NHP carcasses collected throughout Minas Gerais in 2017 and 2018. We demonstrated the wide occurrence of YFV-infected NHP, including the viral persistence during the non-epidemic dry season of 2017. YFV RNA was detected in NHP carcasses in the urban, urban-rural interface and rural areas. We have also detected new YF cases in 49 municipalities where YF cases have not been previously detected during the outbreaks. Estimates of YFV genomic load in naturally infected NHP carcasses showed high and similar loads in specimens (Alouatta,Callithrix, andCallicebus) collected in rural areas and lower genomic loads in the urban-rural interface and urbanCallithrixspecimens. The presence of YFV inside urban areas poses an imminent risk, although no human case was epidemiologically linked to urban/Aedestransmission during the last outbreaks in Brazil. (AU)

• FAPESP's process: 13/21719-3 - Epidemiological study of dengue (serotypes1-4) in a cohort of São José do Rio Preto, São Paulo, Brazil, during 2014-2018
• Grantee: Maurício Lacerda Nogueira
• Support Opportunities: Research Projects - Thematic Grants