Water free incorporation of shark liver oil into starch microparticles by supercriticalCO(2)impregnation at low temperature

Author(s)

Santos, Debora Nascimento e ^[1] ; Aredo, Victor ^[1] ; Bazito, Reinaldo Camino ^[2] ; de Oliveira, Alessandra Lopes ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Anim Sci & Food Engn FZEA, Dept Food Engn, Nat Prod & High Pressure Technol Lab LTAPPN, BR-13635900 Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Inst Chem IQ, Dept Fundamental Chem, Grp Green & Environm Chem GQVA, Butanta, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF FOOD PROCESS ENGINEERING; v. 43, n. 11 NOV 2020.
• Web of Science Citations: 0

Abstract

Shark liver oil (SLO), rich in squalene, was incorporated into modified starch microparticles (MSMs) by supercritical CO2. This polyunsaturated triterpene presents antitumor and antioxidant activities and, due to the high level of unsaturation in the molecule, it is vulnerable to oxidative attack. This research therefore contributes to the development of methods for impregnating such oil into MSM in order to increase the oil's oxidation stability and prevent its degradation in order to facilitate its use in mixtures with inputs for drugs, foods, and cosmetic. To form SLO-incorporated MSM, a physical mixture composed of 44% of two MSM with different degrees of hydrophobicity, 11% SLO, 1% lecithin, and 44% ethanol were treated with supercritical CO(2)at 120 bar, 40, and 60 degrees C. The SLO-incorporated MSM preserved MSM morphology and exhibited good incorporation of SLO (74-82%), a high degree of solubilization in water (78-86%), and a low hygroscopicity (4-9%). Stability evaluation revealed that the SLO-incorporated MSM of Purity Gum Ultra that was formed at 60 degrees C preserved 44% of the initial squalene content. Accordingly, supercritical impregnation represents a practical alternative for SLO impregnation since it is a quick method that does not use water in its formulation and can be done at low temperature. Practical Applications The improvement of drug and food inputs that are thermosensitive in nature and/or of low polarity can be produced by using low-cost wall material that can make the process more cost effective and more accessible. Particle formation by supercritical CO(2)impregnation is a green and nonaggressive process for bioactive compounds that allows the design of innovative food products. Moreover, the fact that the use of modified starches for wall materials, which are already used safely by the food and pharmaceutical industries, makes the process and derived products more reliable. In this sense, the incorporation of shark liver oil (rich is squalene) in modified starch microparticles by supercritical impregnation could be a practical alternative for powdered food industries. (AU)

• FAPESP's process: 12/06981-0 - Study of the particle formation and encapsulation using RESS (Rapid Expansion of Supercritical Solutions)
• Grantee: Alessandra Lopes de Oliveira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/00467-3 - MICROENCAPSULATION OF SQUALENE FROM DOGFISH LIVER OIL THROUGH PRECIPITATION WITH SUPERCRITICAL CO2 USING THE RESS TECHNIQUE
• Grantee: Débora Nascimento e Santos
• Support Opportunities: Scholarships in Brazil - Doctorate