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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genome duplication in Leishmania major relies on persistent subtelomeric DNA replication

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Author(s):
Damasceno, Jeziel Dener [1] ; Marques, Catarina A. [1] ; Beraldi, Dario [1] ; Crouch, Kathryn [1] ; Lapsley, Craig [1] ; Obonaga, Ricardo [2] ; Tosi, Luiz R. O. [2] ; McCulloch, Richard [1]
Total Authors: 8
Affiliation:
[1] Univ Glasgow, Wellcome Ctr Integrat Parasitol, Inst Infect Immun & Inflammat, Glasgow, Lanark - Scotland
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Cell & Mol Biol, Ribeirao Preto - Brazil
Total Affiliations: 2
Document type: Journal article
Source: eLIFE; v. 9, SEP 8 2020.
Web of Science Citations: 2
Abstract

DNA replication is needed to duplicate a cell's genome in S phase and segregate it during cell division. Previous work in Leishmania detected DNA replication initiation at just a single region in each chromosome, an organisation predicted to be insufficient for complete genome duplication within S phase. Here, we show that acetylated histone H3 (AcH3), base J and a kinetochore factor co-localise in each chromosome at only a single locus, which corresponds with previously mapped DNA replication initiation regions and is demarcated by localised G/T skew and G4 patterns. In addition, we describe previously undetected subtelomeric DNA replication in G2/M and G1-phase-enriched cells. Finally, we show that subtelomeric DNA replication, unlike chromosome-internal DNA replication, is sensitive to hydroxyurea and dependent on 9-1-1 activity. These findings indicate that Leishmania's genome duplication programme employs subtelomeric DNA replication initiation, possibly extending beyond S phase, to support predominantly chromosome-internal DNA replication initiation within S phase. (AU)

FAPESP's process: 17/07092-9 - How do checkpoint proteins Rad9 and Hus1 act to maintain genome stability in the protozoan Leishmania major?
Grantee:Luiz Ricardo Orsini Tosi
Support Opportunities: Regular Research Grants