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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Mesenchymal Stromal Cells Derived from Bone Marrow and Adipose Tissue: Isolation, Culture, Characterization and Differentiation

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Freitas, Gileade P. [1] ; Souza, Alann T. P. [1] ; Lopes, Helena B. [1] ; Trevisan, Rayana L. B. [1] ; Oliveira, Fabiola S. [1] ; Fernandes, Roger R. [1] ; Ferreira, Fernanda U. [2] ; Rose, Felipe A. [2] ; Beloti, Marcio M. [1] ; Rosa, Adalberto L. [1]
Total Authors: 10
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Bone Res Lab, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Hemotherapy Ctr Ribeirao Preto, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: BIO-PROTOCOL; v. 10, n. 4 FEB 20 2020.
Web of Science Citations: 1

Since their discovery, mesenchymal stromal cells (MSCs) have received a lot of attention, mainly due to their self-renewal potential and multilineage differentiation capacity. For these reasons, MSCs are a useful tool in cell biology and regenerative medicine. In this article, we describe protocols to isolate MSCs from bone marrow (BM-MSCs) and adipose tissues (AT-MSCs), and methods to culture, characterize, and differentiate MSCs into osteoblasts, adipocytes, and chondrocytes. After the harvesting of cells from bone marrow by flushing the femoral diaphysis and enzymatic digestion of abdominal and inguinal adipose tissues, MSCs are selected by their adherence to the plastic tissue culture dish. Within 7 days, MSCs reach 70% confluence and are ready to be used in subsequent experiments. The protocols described here are easy to perform, cost-efficient, require minimal time, and yield a cell population rich in MSCs. (AU)

FAPESP's process: 18/17356-6 - Effect of titanium surface with nanotopography on the interaction between osteoblasts and osteoclasts
Grantee:Márcio Mateus Beloti
Support type: Regular Research Grants
FAPESP's process: 17/12622-7 - Cell therapy: potential of mesenchymal stem cells, VEGF-A and BMP-9 to regenerate bone tissue
Grantee:Adalberto Luiz Rosa
Support type: Research Projects - Thematic Grants