Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Main bacterial species causing clinical disease in ornamental freshwater fish in Brazil

Full text
Author(s):
Cardoso, Pedro H. M. [1] ; Moreno, Luisa Z. [1, 2] ; de Oliveira, Carolina H. [1] ; Gomes, Vasco T. M. [1] ; Silva, Ana Paula S. [1] ; Barbosa, Mikaela R. F. [3] ; Sato, Maria Ines Z. [3] ; Balian, Simone C. [1] ; Moreno, Andrea M. [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Fac Med Vet, Dept Med Vet Prevent & Saude Anim, BR-05508270 Sao Paulo - Brazil
[2] Ctr Univ Max Planck UniMax, Indaiatuba - Brazil
[3] Companhia Ambiental Estado Sao Paulo CETESB, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Folia Microbiologica; v. 66, n. 2 NOV 2020.
Web of Science Citations: 0
Abstract

Bacterial diseases are common in ornamental fish, more frequently associated with ubiquitous bacteria from the aquarium environment. The disease can lead to fish mortality and cause high economic losses if not rapidly controlled. The aim of this study was to identify the main causative bacterial agents of infection in ornamental fish with different clinical signs. A total of 126 freshwater fish, from 12 families and 38 species, with clinical signs were collected in a wholesaler in Sao Paulo, SP, Brazil. Samples were taken from the eye, skin ulcers, kidneys, and gills, plated on MacConkey, CHROMagar Orientation, and blood agar and incubated under aerobic and anaerobic conditions. Bacterial identification was performed by MALDI-TOF mass spectrometry. From the 126 studied animals, 112 were positive for bacterial isolation. Among the positive animals, 32.1% presented infection caused by a single bacterial species, while in the remaining 67.9%, two to six different bacterial species were identified. A total of 259 bacterial strains were obtained and classified among 46 bacterial species. The species of higher frequency were Aeromonas veronii (26.3%), Aeromonas hydrophilla (16.2%), Shewanella putrefaciens (7.3%), Citrobacter freundii (8.1%), Vibrio albensis (5.8%), and Klebsiella pneumoniae (4.2%). MALDI-TOF MS showed to be a rapid method for diagnosis of bacterial disease outbreaks in ornamental fish establishments. (AU)

FAPESP's process: 16/25745-7 - Virulence assessment and phenotypic and genomic characterization of Corynebacterium spp. isolated from sow's urinary tract infection
Grantee:Luisa Zanolli Moreno
Support Opportunities: Scholarships in Brazil - Post-Doctoral