Study of different routes of immunization using outer membrane vesicles of Neisseria meningitidis B and comparison of two adjuvants

Author(s)

Brito, Luciana T. ^{[1, 2]} ; Rinaldi, Fabiana M. ^{[1, 2]} ; Gaspar, Emanuelle B. ^[3] ; Correa, Victor Araujo ^{[1, 2]} ; Goncalves, Caroline A. ^[1] ; Portilho, Amanda Izeli ^{[1, 2]} ; de Lima, Gabriela Trzewikoswki ^{[1, 2]} ; De Gaspari, Elizabeth ^{[1, 2]}

Affiliation

^[1] Adolfo Lutz Inst, Dept Immunol, Dr Arnaldo Av 355, 11 Floor, Room 1116, BR-01246902 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Postgrad Program Interun Biotechnol, Sao Paulo, SP - Brazil

^[3] Embrapa Southern Reg Anim Husb, Bage, RS - Brazil

• Document type: Journal article
• Source: Vaccine; v. 38, n. 48, p. 7674-7682, NOV 10 2020.
• Web of Science Citations: 0

Abstract

Outer membrane vesicles (OMVs) of Neisseria meningitidis contain important antigens to trigger an immune response against meningococci and have been studied as vaccines compounds. The immune response to a vaccine may be affected by its constitution and route of administration. Therefore, Swiss mice were immunized by different routes with OMVs of N. meningitidis B with dimethyl dioctadecyl ammonium bromide in bilayer fragments (DDA-BF) or aluminum hydroxide (AH) as adjuvants. The adjuvants and different routes were compared regarding the immune responses by ELISA, western blot, delayed type hypersensitivity (DTH) and histopathologic analysis. The antigenic preparation generated humoral and cellular immune responses. In quantitative analyzes, in general, AH was superior to DDA-BF. However, analysis such as IgG avidity index, bactericidal activity and immunoblot, revealed no important differences regarding the adjuvant or route of immunization. Regarding the parameters tested, it was not possible to define a superiority between the adjuvants and routes of immunization proposed by this study. (C) 2020 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 12/15568-0 - Study of the immunogenicity of outer membrane antigens of Neisseria meningitidis and interaction with cationic lipids: evaluation of different immunization schedules in different strains of mice
• Grantee: Elizabeth Natal de Gaspari
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/11147-2 - Study of the immunogenicity of outer membrane antigens of Neisseria meningitidis and interaction with cationic lipids: evaluation of different immunization schedules in different strains of mice
• Grantee: Luciana Tendolini Brito
• Support Opportunities: Scholarships in Brazil - Master