OsteoBLAST: Computational Routine of Global Molecular Analysis Applied to Biomaterials Development

Author(s)

Ferreira, Marcel Rodrigues ^[1] ; Milani, Renato ^[2] ; Rangel, Elidiane C. ^[3] ; Peppelenbosch, Maikel ^[4] ; Zambuzzi, Willian ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Chem & Biochem, Sao Paulo - Brazil

^[2] Univ Campinas UNICAMP, Inst Biol, Bioquim & Biol Tecidual, Sao Paulo - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Sci & Technol, Sao Paulo - Brazil

^[4] Erasmus MC, Univ Med Ctr Rotterdam, Dept Gastroenterol & Hepatol, Rotterdam - Netherlands

• Document type: Journal article
• Source: FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY; v. 8, OCT 8 2020.
• Web of Science Citations: 0

Abstract

For bone purposes, surface modifications are a common trend in biomaterials research aiming to reduce the time necessary for osteointegration, culminating in faster recovery of patients. In this scenario, analysis of intracellular signaling pathways have emerged as an important and reliable strategy to predict biological responses from in vitro approaches. We have combined global analysis of intracellular protein phosphorylation, systems biology and bioinformatics into an early biomaterial analysis routine called OsteoBLAST. We employed the routine as follows: the PamChip tyrosine kinase assay was applied to mesenchymal stem cells grown on three distinct titanium surfaces: machined, dual acid-etched and nanoHA. Then, OsteoBLAST was able to identify the most reliable spots to further obtain the differential kinome profile and finally to allow a comparison among the different surfaces. Thereafter, NetworKIN, STRING, and Cytoscape were used to build and analyze a supramolecular protein-protein interaction network, and DAVID tools identified biological signatures in the differential kinome for each surface. (AU)

• FAPESP's process: 18/05731-7 - Lyophilized PRP associated with nano-hydroxyapatite in cell performance and bone regeneration
• Grantee: Marcel Rodrigues Ferreira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/22689-3 - Microvesicle/proteins-mediated paracrine signaling among bone and endothelial cells during bone development and regeneration
• Grantee: Willian Fernando Zambuzzi
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 15/03639-8 - Global signal transduction involved in the immediate response to the two surfaces of titanium alloys: building OsteoBLAST database
• Grantee: Marcel Rodrigues Ferreira
• Support Opportunities: Scholarships in Brazil - Master