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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Ovarian follicular dynamics, progesterone concentrations, pregnancy rates and transcriptional patterns in Bos indicus females with a high or low antral follicle count

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Author(s):
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de Lima, Marina Amaro [1] ; Morotti, Fabio [2, 3] ; Bayeux, Bernardo Marcozzi [4] ; de Rezende, Romulo Germano [4] ; Botigelli, Ramon Cesar [5] ; Camara De Bem, Tiago Henrique [1] ; Fontes, Patricia Kubo [5] ; Gouveia Nogueira, Marcelo Fabio [6] ; Meirelles, Flavio Vieira [1] ; Baruselli, Pietro Sampaio [4] ; da Silveira, Juliano Coelho [1] ; Perecin, Felipe [1] ; Seneda, Marcelo Marcondes [2]
Total Authors: 13
Affiliation:
[1] FZEA USP, Dept Vet Med, Pirassununga, SP - Brazil
[2] State Univ Londrina UEL, Anim Reprod & Biotechnol Lab, Londrina, Parana - Brazil
[3] Univ Estadual Londrina, CCA, DCV, Lab Reprod Anim, Cx Postal 10-011, BR-86057970 Londrina, Parana - Brazil
[4] FMVZ USP, Dept Anim Reprod, Sao Paulo, SP - Brazil
[5] IBB UNESP, Inst Biosci, Botucatu, SP - Brazil
[6] FCL UNESP, Dept Biol Sci, Assis, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 10, n. 1 NOV 11 2020.
Web of Science Citations: 0
Abstract

We evaluated the effect of the antral follicle count (AFC) on ovarian follicular dynamics, pregnancy rates, progesterone concentrations, and transcriptional patterns of genes in Nelore cattle (Bos taurus indicus) after a timed artificial insemination (TAI) programme. Cows were separated based on the AFC, and those with a high AFC showed a larger (P<0.0001) ovarian diameter and area than those with a very low AFC. Females with a very low AFC exhibited a larger (P<0.01) diameter of the dominant follicle at TAI (13.6 +/- 0.3 vs. 12.2 +/- 0.4 mm) and a tendency (P=0.06) to have different serum progesterone concentrations (2.9 +/- 0.3 vs. 2.1 +/- 0.3 ng/mL; on day 18, considering day 0 as the beginning of the synchronization protocol) than those with a high AFC. The pregnancy rate was higher (P <= 0.05) in animals with a very low (57.9%) and low (53.1%) AFC than in those with a high AFC (45.2%). The expression of genes related to intercellular communication, meiotic control, epigenetic modulation, cell division, follicular growth, cell maintenance, steroidogenesis and cellular stress response was assessed on day 5. In females with a low AFC, 8 and 21 genes in oocytes and cumulus cells, respectively, were upregulated (P<0.05), while 3 and 6 genes in oocytes and cumulus cells, respectively, were downregulated. The results described here will help elucidate the differences in ovarian physiology and the reproductive success of Bos indicus females with a low or high AFC. (AU)

FAPESP's process: 18/13155-6 - Mechanisms of lipid accumulation in oocytes under metabolic altered environments: in vitro maturation and obesity
Grantee:Felipe Perecin
Support type: Regular Research Grants
FAPESP's process: 12/50533-2 - GIFT: genomic improvement of fertilization traits in Danish and Brazilian cattle
Grantee:Marcelo Fábio Gouveia Nogueira
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/22887-0 - Cell-secreted vesicles containing miRNAs modulate epigenetic changes during in vitro culture of bovine gametes and embryos
Grantee:Juliano Coelho da Silveira
Support type: Research Grants - Young Investigators Grants