| Full text | |
| Author(s): Show less - |
Schiavetto, Camila Marques
[1]
;
de Abreu, Priscila Marinho
[2]
;
von Zeidler, Sandra Ventorin
[2]
;
de Jesus, Lais Machado
[1]
;
Carvalho, Raiany Santos
[3]
;
Cirino, Maria Thereza
[1]
;
Carloni, Adriana Cruvinel
[1]
;
Oliveira, Cristina
[1]
;
Scapulatempo-Neto, Cristovam
[1, 4]
;
de Almeida, Gisele Caravina
[5, 4]
;
de Menezes, Nei Soares
[5]
;
Carvalho, Andre Lopes
[1]
;
Reis, Rui Manuel
[6, 7, 1]
;
de Carvalho, Ana Carolina
[1]
Total Authors: 14
|
| Affiliation: | [1] Barretos Canc Hosp, Mol Oncol Res Ctr, Rua Antenor Duarte Vilela 1331, BR-14784400 Barretos, SP - Brazil
[2] Univ Fed Espirito Santo, Programa Posgrad Biotecnol, Ctr Ciencias Saude, Vitoria, ES - Brazil
[3] Barretos Canc Hosp, Res Support Ctr, Sao Paulo - Brazil
[4] Diagnost Amer DASA, Pathol & Mol Diagnost Serv, Sao Paulo - Brazil
[5] Barretos Canc Hosp, Dept Pathol, Sao Paulo - Brazil
[6] ICVS 3Bs PT Govt Associate Lab, Braga - Portugal
[7] Univ Minho, Sch Med, Life & Hlth Sci Res Inst ICVS, Braga - Portugal
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | MOLECULAR DIAGNOSIS & THERAPY; v. 25, n. 1 NOV 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Introduction High-risk human papillomavirus infection impacts staging and prognosis of oropharyngeal squamous cell carcinomas (OPSCCs). Determination of HPV status in tumor tissue by p16-immunohistochemistry (p16-IHC) can be challenging; therefore, complementary methodologies could be useful in a clinical setting. Objective To test for accuracy and clinical relevance of HPV-DNA detection in formalin-fixed and paraffin-embedded (FFPE) tumor samples by droplet digital PCR (ddPCR). Materials and Methods Fifty OPSCCs were tested for p16-IHC status followed by HPV-16/18 DNA detection/quantification in FFPE-recovered DNA using ddPCR. Accuracy for HPV status determination and association with patient information were also evaluated. Results 32.0% (16/50) of the cases were p16-IHC positive (p16 +), 42.0% (21/50) had detectable levels of HPV-16 DNA, and none were positive for HPV-18 DNA. A higher median viral load of HPV-16 DNA was observed in p16 + cases (p < 0.0001). Concordance between p16-IHC and HPV-16 DNA ranged from 78.0 to 86.0% and accuracy rates were between 78.0 and 86.0%. P16-IHC and HPV-16 DNA detection was associated with gender, smoking status, and tumor subsite, while only HPV-16 DNA was associated with cT stage. The combination of HPV positivity by p16-IHC and ddPCR showed higher overall survival rates in comparison with p16 + /HPV-DNA- and p16 - /HPV-DNA- results. Conclusions Type-specific HPV-DNA detection by ddPCR is highly specific but moderately sensitive for the determination of HPV status and showed clinical relevance, mainly when associated with p16-IHC status. Results highlight the importance of performing HPV-DNA testing in combination with p16-IHC for proper identification of HPV-associated OPSCC and to improve clinical management of OPSCC patients. (AU) | |
| FAPESP's process: | 15/01286-0 - Characterization of genetic and epigenetic alterations in specific genes in HPV-positive and HPV-negative tumors of patients with head and neck squamous cell carcinoma |
| Grantee: | André Lopes Carvalho |
| Support Opportunities: | Regular Research Grants |