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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Liquid biopsy based on small extracellular vesicles predicts chemotherapy response of canine multicentric lymphomas

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Author(s):
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Garnica, Taismara K. [1] ; Lesbon, Jessika C. C. [1] ; Avila, Ana C. F. C. M. [2] ; Rochetti, Arina L. [1] ; Matiz, Oscar R. S. [3] ; Ribeiro, Roana C. S. [3] ; Zoppa, Aline [4] ; Nishiya, Adriana T. [4] ; Costa, Mirela T. [3] ; de Nardi, Andrigo B. [3] ; Argyle, David J. [5, 6] ; Strefezzi, Ricardo F. [1] ; Silveira, Juliano C. [2] ; Fukumasu, Heidge [1]
Total Authors: 14
Affiliation:
[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Lab Comparat & Translat Oncol LOCT, Pirassununga - Brazil
[2] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Lab Mol Morphophysiol & Dev LMMD, Pirassununga - Brazil
[3] Sao Paulo State Univ Julio de Mesquita Filho UNES, Coll Agr & Vet Sci, Clin Vet Dept, Jaboticabal, SP - Brazil
[4] Anhembi Morumbi Univ, Vet Hosp Anhembi Morumbi, Sao Paulo, SP - Brazil
[5] Univ Edinburgh, Royal Dick Sch Vet Studies, Edinburgh, Midlothian - Scotland
[6] Univ Edinburgh, Roslin Inst, Edinburgh, Midlothian - Scotland
Total Affiliations: 6
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 10, n. 1 NOV 23 2020.
Web of Science Citations: 1
Abstract

Lymphoma is the most common type of canine hematological malignancy where the multicentric (cMCL) form accounts for 75% of all cases. The standard treatment is the CHOP chemotherapy protocols that include cyclophosphamide, doxorubicin, vincristine and prednisone, where the majority of dogs achieve complete/partial response; however, it is very important to predict non-responsive cases to improve treatment and to develop new targeted therapies. Here we evaluate a liquid biopsy approach based on serum Small Extracellular Vesicles enriched for exosomes (SEVs) to predict cMCL chemotherapy response. Nineteen dogs at the end of the 19-week chemotherapy protocol (8 Complete Response and 11 Progressive Disease) were evaluated for serum SEVs size, concentration and screened for 95 oncomirs. PD patients had higher SEVs concentration at the diagnosis than CR patients (P=0.034). The ROC curve was significant for SEVs concentration to predict the response to CHOP (AUC=0.8011, P=0.0287). A potential molecular signature based on oncomirs from SEVs (caf-miR-205, caf-miR-222, caf-mir-20a and caf-miR-93) is proposed. To the best of our knowledge, this is the first study demonstrating the potential of a liquid biopsy based on SEVs and their miRNAs content to predict the outcome of chemotherapy for canine multicentric lymphomas. (AU)

FAPESP's process: 14/02493-7 - Mammary tumors of dogs and the cancer stem cell theory: a comparative and translational approach
Grantee:Heidge Fukumasu
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 15/21829-9 - Multi-user equipment approved in grant 2014/22887-0: Nanosight LM10
Grantee:Juliano Coelho da Silveira
Support Opportunities: Multi-user Equipment Program
FAPESP's process: 17/15406-3 - Extracellular vesicles as potencial predictive marker in canine multicentric lymphoma
Grantee:Taismara Kustro Garnica
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 14/22887-0 - Cell-secreted vesicles containing miRNAs modulate epigenetic changes during in vitro culture of bovine gametes and embryos
Grantee:Juliano Coelho da Silveira
Support Opportunities: Research Grants - Young Investigators Grants