The relevance of enzyme specificity for coenzymes and the presence of 6-phosphogluconate dehydrogenase for polyhydroxyalkanoates production in the metabolism of Pseudomonas sp. LFM046

Author(s)

Cardinali-Rezende, Juliana ^{[1, 2]} ; Di Genova, Alex ^[3] ; Nahat, Rafael A. T. P. S. ^[1] ; Steinbuechel, Alexander ^{[2, 4]} ; Sagot, Marie-France ^[3] ; Costa, Rafael S. ^{[5, 6]} ; Oliveira, Henrique C. ^[1] ; Taciro, Marilda K. ^[1] ; Silva, Luiziana F. ^[1] ; Gomez, Jose Gregorio C. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Bioprod Lab, Prof Lineu Prestes Ave 1374, Sao Paulo - Brazil

^[2] Westfalische Wilhelms Univ Munster, Inst Molekulore Mikrobiol & Biotechnol, Corrensstr 3, D-48149 Munster - Germany

^[3] Univ Lyon, Univ Lyon 1, CNRS, ERABLE Team, Inria Grenoble Rhone Alpes, Lab Biometrie & Biol Evolut UMR 5558, F-69122 Villeurbanne - France

^[4] King Abdulaziz Univ, Environm Sci Dept, Jeddah - Saudi Arabia

^[5] Univ Lisbon, Inst Super Tecn, IDMEC, Av Rovisco Pais 1, P-1049001 Lisbon - Portugal

^[6] Univ Nova Lisboa, Fac Sci & Technol, Dept Chem, REQUIMTE, LAQV, Campus Caparica, P-2829516 Caparica - Portugal

• Document type: Journal article
• Source: International Journal of Biological Macromolecules; v. 163, p. 240-250, NOV 15 2020.
• Web of Science Citations: 0

Abstract

Reconstruction of genome-based metabolic model is a useful approach for the assessment of metabolic pathways, genes and proteins involved in the environmental fitness capabilities or pathogenic potential as well as for biotechnological processes development. Pseudomonas sp. LFM046 was selected as a good polyhydroxyalkanoates (PHA) producer from carbohydrates and plant oils. Its complete genome sequence and metabolic model were obtained. Analysis revealed that the gnd gene, encoding 6-phosphogluconate dehydrogenase, is absent in Pseudomonas sp. LFM046 genome. In order to improve the knowledge about LFM046 metabolism, the coenzyme specificities of different enzymes was evaluated. Furthermore, the heterologous expression of gnd genes from Pseudomonas putida KT2440 (NAD(+) dependent) and Escherichia coli MG1655 (NADP(+) dependent) in LFM046 was carried out and provoke a delay on cell growth and a reduction in PHA yield, respectively. The results indicate that the adjustment in cyclic Entner-Doudoroff pathway may be an interesting strategy for it and other bacteria to simultaneously meet divergent cell needs during cultivation phases of growth and PHA production. (C) 2020 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 10/51692-1 - Metabolic engineering applied to biopolymer production
• Grantee: Marilda Keico Taciro
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants
• FAPESP's process: 14/08061-1 - Genomic scale metabolic network reconstruction and improvement of polyhydroxyalkanoates production in Pseudomonas sp. (LFM046)
• Grantee: Juliana Cardinali Rezende
• Support Opportunities: Scholarships in Brazil - Post-Doctoral