Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Sequential design of experiments approach for the multiproduct analysis of cholesterol-lowering drugs by ultra-high-performance supercritical fluid chromatography

Full text
Author(s):
Santana, Igor Miranda [1] ; Jardim, Isabel Cristina Sales Fontes [1] ; Breitkreitz, Marcia Cristina [1]
Total Authors: 3
Affiliation:
[1] Univ Estadual Campinas, Inst Chem, Dept Analyt Chem, Campinas, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF SEPARATION SCIENCE; OCT 2020.
Web of Science Citations: 0
Abstract

A multiproduct approach toward method development is presented for a fast and reliable analysis of the eight most important cholesterol-lowering drugs via ultra-high-performance supercritical fluid chromatography. A two-step approach based on design of experiments was applied: (1) selection of the stationary phase, organic modifier, and diluent in the mobile phase through a multilevel categorical design and (2) optimization of the elution strength by varying the pressure, temperature, and gradient using a central composite design. Finally, the flow rate was adjusted. The first design selected UPC(2)Torus 1-AA as the column, ethanol:water as the organic modifier, and acetonitrile:ethanol 3:2 v/v as the diluent. The results led to a pressure, column temperature, and gradient elution of 14.83 MPa, 42 degrees C, and 5-15.5% of ethanol:water in CO2, respectively. The flow rate was set at 1.8 mL/min, providing a total analysis time of 4 min. This multiproduct method was validated and applied to 11 different commercial products available in the Brazilian market, and it was found to be accurate, withr> 0.990, recoveries between 95 and 105%, and precision not higher than 5.4%. Therefore, this method was shown to be a greener alternative for the analysis of these pharmaceuticals. (AU)

FAPESP's process: 16/05636-9 - Development of innovative analytical methods for analytes of pharmaceutical interest based on ultra-high supercritical fluid chromatography associated with chemometrics tools
Grantee:Márcia Cristina Breitkreitz
Support Opportunities: Regular Research Grants