| Full text | |
| Author(s): Show less - |
Rosa-Fernandes, Livia
[1, 2]
;
Barbosa, Raquel Hora
[2, 3, 4, 5]
;
dos Santos, Maria Luiza B.
[4]
;
Angeli, Claudia B.
[2]
;
Silva, Thiago P.
[6]
;
Melo, Rossana C. N.
[6]
;
de Oliveira, Gilberto Santos
[2]
;
Lemos, Bernardo
[3]
;
Van Eyk, Jennifer E.
[7]
;
Larsen, Martin R.
[1]
;
Cardoso, Claudete Araujo
[4]
;
Palmisano, Giuseppe
[2]
Total Authors: 12
|
| Affiliation: | [1] Univ Southern Denmark, Dept Biochem & Mol Biol, Odense - Denmark
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, GlycoProte Lab, Sao Paulo - Brazil
[3] Harvard Sch Publ Hlth, Mol & Integrat Physiol Sci Program, Dept Environm Hlth, Boston, MA - USA
[4] Fluminense Fed Univ, Fac Med, Maternal & Child Dept, Rio De Janeiro - Brazil
[5] Natl Canc Inst, Genet Program, Rio De Janeiro - Brazil
[6] Univ Fed Juiz de Fora, Dept Biol, Lab Cellular Biol, Juiz De Fora, MG - Brazil
[7] Cedars Sinai Med Ctr, Adv Clin BioSyst Res Inst, Cedars Sinai Precis Biomarker Labs, Barbra Streisand Womens Heart Ctr, Los Angeles, CA - USA
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | JOURNAL OF PROTEOME RESEARCH; v. 19, n. 11, p. 4496-4515, NOV 6 2020. |
| Web of Science Citations: | 1 |
| Abstract | |
Congenital Zika syndrome was first described due to increased incidence of congenital abnormalities associated with Zika virus (ZIKV) infection. Since the eye develops as part of the embryo central nervous system (CNS) structure, it becomes a specialized compartment able to display symptoms of neurodegenerative diseases and has been proposed as a noninvasive approach to the early diagnosis of neurological diseases. Ocular lesions result from defects that occurred during embryogenesis and can become apparent in newborns exposed to ZIKV. Furthermore, the absence of microcephaly cannot exclude the occurrence of ocular lesions and other CNS manifestations. Considering the need for surveillance of newborns and infants with possible congenital exposure, we developed a method termed cellular imprinting proteomic assay (CImPA) to evaluate the ocular surface proteome specific to infants exposed to ZIKV during gestation compared to nonexposure. CImPA combines surface cells and fluid capture using membrane disks and a large-scale quantitative proteomics approach, which allowed the first-time report of molecular alterations such as neutrophil degranulation, cell death signaling, ocular and neurological pathways, which are associated with ZIKV infection with and without the development of congenital Zika syndrome, CZS. Particularly, infants exposed to ZIKV during gestation and without early clinical symptoms could be detected using the CImPA method. Lastly, this methodology has broad applicability as it could be translated in the study of several neurological diseases to identify novel diagnostic biomarkers. Data are available via ProteomeXchange with identifier PXD014038. (AU) | |
| FAPESP's process: | 18/15549-1 - Post-translational modifications in Chagas Disease biological processes and diagnostics: novel methodological approaches and biological applications |
| Grantee: | Giuseppe Palmisano |
| Support Opportunities: | Research Grants - Young Investigators Grants - Phase 2 |
| FAPESP's process: | 18/18257-1 - Multi-user equipment approved in grant 14/06863-3: HPLC system configured for analysis of carbohydrates, amino acidis, peptides and glycoproteins |
| Grantee: | Giuseppe Palmisano |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 14/06863-3 - Post-translational modifications in cancer and parasite infection diagnosis: methodological approaches and biological implications |
| Grantee: | Giuseppe Palmisano |
| Support Opportunities: | Research Grants - Young Investigators Grants |