Controlling the Surface Functionalization of Ultrasmall Gold Nanoparticles by Sequence-Defined Macromolecules

Author(s):Show less -

van Der Meer, Selina Beatrice ^{[1, 2]} ; Seiler, Theresa ^[3] ; Buchmann, Christin ^[3] ; Partalidou, Georgia ^[3] ; Boden, Sophia ^[3] ; Loza, Kateryna ^{[1, 2]} ; Heggen, Marc ^[4] ; Linders, Jurgen ^{[2, 5]} ; Prymak, Oleg ^{[1, 2]} ; Oliveira, Cristiano L. P. ^[6] ; Hartmann, Laura ^[3] ; Epple, Matthias ^{[1, 2]}

Affiliation

^[1] Univ Duisburg Essen, Inorgan Chem, Univ Str 5-7, D-45117 Essen - Germany

^[2] Univ Duisburg Essen, Ctr Nanointegrat Duisburg Essen CeNIDE, Univ Str 5-7, D-45117 Essen - Germany

^[3] Heinrich Heine Univ Dusseldorf, Organ Chem & Macromol Chem, Univ Str 1, D-40225 Dusseldorf - Germany

^[4] Forschungszentrum Julich, Ernst Ruska Ctr Microscopy & Spect Electrons, D-52425 Julich - Germany

^[5] Univ Duisburg Essen, Phys Chem, Univ Str 5-7, D-45117 Essen - Germany

^[6] Univ Sao Paulo, Inst Phys, Rua Matao 1371, BR-05508090 Sao Paulo - Brazil

• Document type: Journal article
• Source: CHEMISTRY-A EUROPEAN JOURNAL; v. 27, n. 4 DEC 2020.
• Web of Science Citations: 0

Abstract

Ultrasmall gold nanoparticles (diameter about 2 nm) were surface-functionalized with cysteine-carrying precision macromolecules. These consisted of sequence-defined oligo(amidoamine)s (OAAs) with either two or six cysteine molecules for binding to the gold surface and either with or without a PEG chain (3400 Da). They were characterized by H-1 NMR spectroscopy, H-1 NMR diffusion-ordered spectroscopy (DOSY), small-angle X-ray scattering (SAXS), and high-resolution transmission electron microscopy. The number of precision macromolecules per nanoparticle was determined after fluorescent labeling by UV spectroscopy and also by quantitative H-1 NMR spectroscopy. Each nanoparticle carried between 40 and 100 OAA ligands, depending on the number of cysteine units per OAA. The footprint of each ligand was about 0.074 nm(2) per cysteine molecule. OAAs are well suited to stabilize ultrasmall gold nanoparticles by selective surface conjugation and can be used to selectively cover their surface. The presence of the PEG chain considerably increased the hydrodynamic diameter of both dissolved macromolecules and macromolecule-conjugated gold nanoparticles. (AU)

• FAPESP's process: 16/24531-3 - Structural and biophysics properties of native and modified lipoproteins
• Grantee: Antonio Martins Figueiredo Neto
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/16092-5 - Investigating colloidal systems by scattering methods
• Grantee: Cristiano Luis Pinto de Oliveira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/06750-8 - Probing the non-covalent interaction of ultrasmall gold nanoparticles with selected proteins
• Grantee: Cristiano Luis Pinto de Oliveira
• Support Opportunities: Regular Research Grants