Structural and biophysics properties of native and modified lipoproteins

• Grant number: 16/24531-3
• Support Opportunities: Research Projects - Thematic Grants
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Antonio Martins Figueiredo Neto
• Grantee: Antonio Martins Figueiredo Neto
• Host Institution: Instituto de Física (IF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Pesquisadores principais: Cristiano Luis Pinto de Oliveira ; Nágila Raquel Teixeira Damasceno
• Associated researchers: Alessandro Desideri ; Andrew John Masters ; Cassio Alves ; Daniel Erik Otzen ; Erol Akpinar ; Francisco Antonio Helfenstein Fonseca ; Giovanni Barbero ; Jan Skov Pedersen ; Luis Eduardo Soares Netto ; Maria Aparecida Neves Jardini ; Maria Cristina de Oliveira Izar ; Maria Helena Godinho ; Matthias Epple ; Nágila Raquel Teixeira Damasceno ; Nallet ; Prymak Oleg ; Roberto Kopke Salinas ; Samantha Cristina de Pinho ; Silvia Biocca ; Stefan K. Nilsson
• Associated research grant(s): 19/06750-8 - Probing the non-covalent interaction of ultrasmall gold nanoparticles with selected proteins, AP.R SPRINT; 19/00560-2 - A study on lyotropic biaxial chromonic nematic phases via polarising light microscopy, laser conoscopy and small-angle x-ray scattering, AV.EXT; 19/03720-0 - 87th European Atherosclerosis Society Congress, AR.EXT; 17/20992-9 - Multi user equipment approved in the grant 2016/24531-3: upgrade of the X-ray diffractometer of the laboratory, increasing the scattering vector modulus range and versatile source with three wavelengths controlled by software, AP.EMU
• Associated scholarship(s): 23/04315-8 - Role of interaction between Omega-3 and GPR120 in modulating inflammatory, oxidative, and tumor markers in women with Breast Cancer, BP.MS; 22/10121-9 - Quality analysis of LDL in patients during and after SARCOVID-2 infection, BP.PD; 20/05913-8 - Association of omega-3 fatty acids with survival, tumor recurrence and mortality in women with breast cancer, BP.IC; 19/10433-8 - Simulation and Structural Modeling of Oriented Liquid Crystals using Small-Angle Scattering Methodsng, BP.PD; 18/07340-5 - Structural characterisation of lipoproteins, BP.PD

Abstract

This project joins researchers from different areas as Physics, Medicine, Odontology and Nutrition on the description of problems that needs multidisciplinary focus as the investigation of complex fluids and elastomers. Frontier knowledge will be obtained on the relationship of molecular and nanoparticle interactions which are directly related to the complex behavior of magnetic colloids, liquid crystals, elastomers and supramolecular assemblies of biologic and biochemical interest. The obtained results can provide the development of feed protocols and diagnostic methodologies of Arteriosclerosis and its relationship with Periodontitis and Diabetes. The team consists of researchers and collaborators with large experience on multidisciplinary work and joint publications. Several experimental techniques will be used covering linear and non-linear optics, x-ray diffraction and scattering and calorimetry. Our targets are self-assembly multicomponent systems on thermodynamic equilibrium as liquid crystals, magnetic colloids, micelles, protein-surfactant complexes and human lipoproteins and elastomers. In some cases, model systems on controlled conditions will be investigated in order to provide information on the intricate structure-function-dynamics relationship. The study of lipoproteins, on different in vivo conditions, may provide information on the oxidative processes and its implications on Atherosclerosis. The influence of pathologies like diabetes and periodontitis on the lipoproteins morphology and immune response will be investigated before and after specific interventions. The effect of omega-3 will be investigated on clinic trials in order to determine its influence on the health conditions of adults. The influence of periodontitis on the cardiovascular markers of patients with Diabetes Mellitus will be investigated. In addition, the benefits of the intake of biomass like green-banana by those patients will be evaluated and correlated to the characteristics of the lipoproteins. The research will be supported by nutritionist, who will determine the evolution of the lipid profile of each patient as well as the optic and structural properties of its lipoproteins will be determined before and after the intervention. Thermodynamic and structural characterization of amphiphilic systems and protein-surfactant complexes will be performed using calorimetry and spectroscopic techniques. An innovative approach is applied on these investigations and provides the basis for a quantitative description. Investigations on the interactions between lipids and small molecules will be performed. In this type of study it is necessary the determination of the binding affinity, absorption of the molecule on the lipid membrane as well as the induced structural changes on the overall structure. The study of proteins in directly in solution, which can be performed by x-ray scattering and light scattering, will permit the determination of structural parameters of the macromolecules close to native, in vivo, situation. The project proposes the investigation of physical, chemical and biological properties metallic nanoparticles with several sizes and shapes. The nanoparticles nucleation processes will be investigated and correlated to its general properties. One of our goals is to synthetize and characterizes new celluloid based materials with unique optical properties like the selective reflection of light. Those materials may have tunable photonic structure, either by the use of external electric field or temperature variation. Thermotropic liquid crystals molecules will be added to the celluloid based material in order to enhance structural and optical properties. New elastomeric materials will be synthetized based on PU/PBDO chains with and without the incorporation of magnetic nanoparticles. The mechanical and structural characteristics will be investigated with several experimental complementary techniques, in particular SAXS and DLS. (AU)

Scientific publications

(32)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RUKS, TATJANA; LOZA, KATERYNA; HEGGEN, MARC; PRYMAK, OLEG; SEHNEM, ANDRE LUIZ; OLIVEIRA, CRISTIANO L. P.; BAYER, PETER; BEUCK, CHRISTINE; EPPLE, MATTHIAS. Peptide-Conjugated Ultrasmall Gold Nanoparticles (2 nm) for Selective Protein Targeting. ACS APPLIED BIO MATERIALS, v. 4, n. 1, p. 945-965, JAN 18 2021. (19/10433-8, 19/06750-8, 18/16092-5, 16/24531-3)

PAREK, KINNARI; ESPINOSA, DANIEL H. G.; REIS, DENNYS; DE OLIVEIRA, CRISTIANO L. P.; WLYSSES, WAGNER; FIGUEIREDO NETO, ANTONIO MARTINS. Morphological metamorphosis of magnetic nanoparticles due to the presence of rare earth atoms in the spinel structure: From spheres to cubes. Materials Chemistry and Physics, v. 222, p. 217-226, JAN 15 2019. (16/24531-3, 14/50983-3)

DE SOUZA DEGENHARDT, MAXIMILIA F.; VITALE, PHELIPE A. M.; ABIKO, LAYARA A.; ZACHARIAS, MARTIN; SATTLER, MICHAEL; OLIVEIRA, CRISTIANO L. P.; SALINAS, ROBERTO K.. Molecular insights on CALX-CBD12 interdomain dynamics from MD simulations, RDCs, and SAXS. BIOPHYSICAL JOURNAL, v. 120, n. 17, p. 3664-3675, SEP 7 2021. (18/16092-5, 16/24531-3, 19/19968-1, 17/05614-8, 16/07490-1)

AKPINAR, EROL; UYGUR, NAZLI; UYANIK, SEDA; FIGUEIREDO NETO, ANTONIO MARTINS. Effect of surfactant alkyl chain length on lyotropic uniaxial to biaxial cholesteric phase transitions. EUROPEAN PHYSICAL JOURNAL E, v. 42, n. 9, SEP 2019. (16/24531-3, 14/50983-3)

VIANA MIGUEL, MANUELA MARIA; MATHIAS-SANTAMARIA, INGRID FERNANDES; ROSSATO, AMANDA; FERREIRA FERRAZ, LAIS FERNANDA; FIGUEIREDO-NETO, ANTONIO MARTINS; DE MARCO, ANDREA CARVALHO; VIANA CASARIN, RENATO CORREA; WALLET, SHANNON MARGARET; TATAKIS, DIMITRIS N.; MATHIAS, MARCIO ANTONIO; et al. Microcurrent electrotherapy improves palatal wound healing: Randomized clinical trial. Journal of Periodontology, v. 92, n. 2, AUG 2020. (18/03353-5, 18/07340-5, 16/16102-5, 16/07528-9, 16/24531-3, 14/50983-3)

DO PRADO, ALICE HADDAD; DUARTE, JONATAS LOBATO; DI FILIPPO, LEONARDO DELELLO; VICTORELLI, FRANCESCA DAMIANI; DE ABREU FANTINI, MARCIA CARVALHO; PECCININI, ROSANGELA GONACALVES; CHORILLI, MARLUS. Bioadhesive liquid crystal systems for octyl methoxycinnamate skin delivery. JOURNAL OF MOLECULAR LIQUIDS, v. 345, JAN 1 2022. (16/24531-3, 14/22426-2, 17/17844-8)

VAN DER MEER, SELINA BEATRICE; SEILER, THERESA; BUCHMANN, CHRISTIN; PARTALIDOU, GEORGIA; BODEN, SOPHIA; LOZA, KATERYNA; HEGGEN, MARC; LINDERS, JURGEN; PRYMAK, OLEG; OLIVEIRA, CRISTIANO L. P.; et al. Controlling the Surface Functionalization of Ultrasmall Gold Nanoparticles by Sequence-Defined Macromolecules. CHEMISTRY-A EUROPEAN JOURNAL, v. 27, n. 4, DEC 2020. (16/24531-3, 18/16092-5, 19/06750-8)

OSELIERO FILHO, PEDRO LEONIDAS; GERBELLI, BARBARA BIANCA; FORNASIER, FRANCCESCA; CHAVES FILHO, ADRIANO B.; YOSHINAGA, MARCOS YUKIO; MIYAMOTO, SAYURI; MORTARA, LAURA; LACERDA, CAROLINE DUTRA; CUCCOVIA, IOLANDA MIDEA; PIMENTEL, ANDRE SILVA; et al. Structure and Thermotropic Behavior of Bovine- and Porcine-Derived Exogenous Lung Surfactants. Langmuir, v. 36, n. 48, p. 14514-14529, DEC 8 2020. (16/24531-3, 19/12301-1, 18/05888-3, 14/50983-3, 18/16092-5)

BARBERO, G.; BATALIOTO, F.; NETO, A. M. FIGUEIREDO; LELIDIS, I.. Deviations from linearity in impedance spectroscopy measurements confirmed by Kramers-Kronig analysis. Electrochimica Acta, v. 397, NOV 20 2021. (16/24531-3, 14/50983-3)

LOTFOLLAHI, ZAHRA; MELLO, ANA P. Q.; FONSECA, FRANCISCO A. H.; MACHADO, LUCIENE O.; MATHIAS, ANDRESSA F.; IZAR, MARIA C.; DAMASCENO, NAGILA R. T.; OLIVEIRA, CRISTIANO L. P.; NETO, ANTONIO M. F.. Changes in lipoproteins associated with lipid-lowering and antiplatelet strategies in patients with acute myocardial infarction. PLoS One, v. 17, n. 8, p. 19-pg., 2022-08-30. (12/51692-7, 16/24531-3, 18/07340-5)

GUSTAVO HENRIQUE FERREIRA GONÇALINHO; GENI RODRIGUES SAMPAIO; ROSANA APARECIDA MANÓLIO SOARES-FREITAS; NÁGILA RAQUEL TEIXEIRA DAMASCENO. Os Ácidos Graxos Poli-insaturados Ômega-6 e Ômega-3 Presentes nas Hemácias Exercem uma Influência Distinta sobre o Tamanho das Partículas de LDL e suas Alterações Estruturais. Arquivos Brasileiros de Cardiologia, v. 120, n. 11, 2023-11-20. (11/12523-2, 16/24531-3)

GARCIA, PAULO RICARDO ABREU FURTADO RICARDO; ARAUJO, WAGNER WLYSSES RODRIGUES; TEOBALDO, GABRIEL BRAGA MARQUES; DE MENEZES, ALAN SILVA; OTUBO, LARISSA; OLIVEIRA, CRISTIANO LUIS PINTO. Synthesis and structural characterization of gold nanorods. INTERNATIONAL NANO LETTERS, JAN 2021. (16/24531-3, 18/16092-5)

MARTINEZ, RENATA MILIANI; OSELIERO FILHO, PEDRO LEONIDAS; GERBELLI, BARBARA BIANCA; MAGALHAES, WAGNER VIDAL; VELASCO, MARIA VALERIA ROBLES; DA SILVA LANNES, SUZANA CAETANO; DE OLIVEIRA, CRISTIANO LUIS PINTO; ROSADO, CATARINA; BABY, ANDRE ROLIM. Influence of the Mixtures of Vegetable Oil and Vitamin E over the Microstructure and Rheology of Organogels. GELS, v. 8, n. 1, JAN 2022. (16/24531-3, 20/02192-8, 19/16169-0, 19/12301-1, 18/05888-3, 20/13204-7)

BATALIOTO, F.; NETO, A. M. FIGUEIREDO; BARBERO, G.. Ions, adsorption and electric response of a ferrofluid cell. Physical Chemistry Chemical Physics, v. 24, n. 5, p. 3400-3409, FEB 2 2022. (16/24531-3, 14/50983-3)

SANTOS, O. R.; REIS, D.; OLIVEIRA-FILHO, A. G.; OLIVEIRA, C. L. P.; FIGUEIREDO NETO, A. M.. tructure and local order of lyotropic cholesteric calamitic phases: The effect of the chiral molecul. JOURNAL OF MOLECULAR LIQUIDS, v. 349, MAR 1 2022. (14/50983-3, 16/24531-3)

BATALIOTO, F.; CHAND, M.; CAMPOS, A. F. C.; DEPEYROT, J.; BARBERO, G.; NETO, A. M. FIGUEIREDO. Nonlinear behavior of the impedance spectrum of a kerosene based ferrofluid. Physical Chemistry Chemical Physics, v. 24, n. 46, p. 7-pg., 2022-11-10. (16/24531-3, 14/50983-3)

GARCIA, PAULO R. A. F.; PRYMAK, OLEG; GRASMIK, VIKTORIA; PAPPERT, KEVIN; WLYSSES, WAGNER; OTUBO, LARISSA; EPPLE, MATTHIAS; OLIVEIRA, CRISTIANO L. P.. An in situ SAXS investigation of the formation of silver nanoparticles and bimetallic silver-gold nanoparticles in controlled wet-chemical reduction synthesis. NANOSCALE ADVANCES, v. 2, n. 1, p. 225-238, JAN 1 2020. (18/16092-5, 16/24531-3)

AKPINAR, EROL; GUNER, EMRE; DEMIR-ORDU, OZNUR; FIGUEIREDO NETO, ANTONIO MARTINS. Effect of head-group size of some tetradecylalkylammonium bromide surfactants on obtaining the lyotropic biaxial nematic phase. EUROPEAN PHYSICAL JOURNAL E, v. 42, n. 4, APR 2019. (16/24531-3, 14/50983-3)

AKPINAR, EROL; FIGUEIREDO NETO, ANTONIO MARTINS. Experimental Conditions for the Stabilization of the Lyotropic Biaxial Nematic Mesophase. CRYSTALS, v. 9, n. 3, MAR 19 2019. (16/24531-3, 14/50983-3)

FERREIRA GONCALINHO, GUSTAVO HENRIQUE; SAMPAIO, GENI RODRIGUES; MANOLIO SOARES-FREITAS, ROSANA APARECIDA; TEIXEIRA DAMASCENO, NAGILA RAQUEL. Omega-3 Fatty Acids in Erythrocyte Membranes as Predictors of Lower Cardiovascular Risk in Adults without Previous Cardiovascular Events. NUTRIENTS, v. 13, n. 6, JUN 2021. (16/24531-3, 11/12523-2)

FRANCO, LEANDRO REZENDE; SEHNEM, ANDRE LUIZ; NETO, ANTONIO MARTINS FIGUEIREDO; COUTINHO, KALINE. Molecular Dynamics Approach to Calculate the Thermodiffusion (Soret and Seebeck) Coefficients of Salts in Aqueous Solutions. JOURNAL OF CHEMICAL THEORY AND COMPUTATION, v. 17, n. 6, p. 3539-3553, JUN 8 2021. (16/24531-3, 19/10433-8, 17/11631-2, 14/50983-3)

DE QUEIROZ MELLO, ANA PAULA; ALBATTARNI, GHADEER; GARCIA ESPINOSA, DANIEL HUMBERTO; REIS, DENNYS; FIGUEIREDO NETO, ANTONIO MARTINS. Structural and Nonlinear Optical Characteristics of In Vitro Glycation of Human Low-Density Lipoprotein, as a Function of Time. Brazilian Journal of Physics, v. 48, n. 6, p. 560-570, DEC 2018. (14/50983-3, 16/24531-3)

SEHNEM, ANDRE LUIZ; JANSSEN, MATHIJS. On the time-dependent electrolyte Seebeck effect. Journal of Chemical Physics, v. 154, n. 16, APR 28 2021. (14/50983-3, 16/24531-3)

PEDROSO, JULIANA DE FATIMA; LOTFOLLAHI, ZAHRA; ALBATTARNI, GHADEER; SCHULZ, MAIARA ARRRUDA; MONTEIRO, ANDREA; SEHNEM, ANDRE LUIZ; GIDLUND, MAGNUS AKE; FIGUEIREDO NETO, ANTONIO MARTINS; NEVES JARDINI, MARIA APARECIDA. Influence of Periodontal Disease on cardiovascular markers in Diabetes Mellitus patients. SCIENTIFIC REPORTS, v. 9, NOV 6 2019. (16/24531-3, 14/50983-3, 18/07340-5)

AKPINAR, EROL; TOPCU, GOKHAN; REIS, DENNYS; FIGUEIREDO NETO, ANTONIO MARTINS. Effect of the anionic azo dye Sunset Yellow in lyotropic mixtures with uniaxial and biaxial nematic phases. JOURNAL OF MOLECULAR LIQUIDS, v. 318, NOV 15 2020. (18/07340-5, 16/24531-3, 14/50983-3)

AKPINAR, EROL; UYGUR, NAZLI; TOPCU, GOKHAN; LAVRENTOVICH, OLEG D.; FIGUEIREDO NETO, ANTONIO MARTINS. Gemini surfactant behavior of conventional surfactant dodecyltrimethylammonium bromide with anionic azo dye Sunset Yellow in aqueous solutions. JOURNAL OF MOLECULAR LIQUIDS, v. 360, p. 13-pg., 2022-06-15. (16/24531-3, 14/50983-3)

GONCALINHO, GUSTAVO HENRIQUE FERREIRA; SAMPAIO, GENI RODRIGUES; SOARES-FREITAS, ROSANA APARECIDA MANOLIO; DAMASCENO, NAGILA RAQUEL TEIXEIRA. Stearic Acid, but not Palmitic Acid, is Associated with Inflammatory and Endothelial Dysfunction Biomarkers in Individuals at Cardiovascular Risk. Arquivos Brasileiros de Cardiologia, v. 120, n. 8, p. 9-pg., 2023-01-01. (11/12523-2, 16/24531-3)

BATALIOTO, F.; BARBERO, G.; CAMPOS, A. F. C.; FIGUEIREDO NETO, A. M.. Free ions in kerosene-based ferrofluid detected by impedance spectroscopy. Physical Chemistry Chemical Physics, v. 23, n. 4, p. 2819-2824, JAN 28 2021. (14/50983-3, 16/24531-3)

GARCIA, PAULO RICARDO ABREU FURTADO RICARDO; ARAUJO, WAGNER WLYSSES RODRIGUES; TEOBALDO, GABRIEL BRAGA MARQUES; DE MENEZES, ALAN SILVA; OTUBO, LARISSA; OLIVEIRA, CRISTIANO LUIS PINTO. Synthesis and structural characterization of gold nanorods. INTERNATIONAL NANO LETTERS, v. 11, n. 1, p. 10-pg., 2021-01-18. (18/16092-5, 16/24531-3)

BATALIOTO, F.; GONCALVES, E. S.; FIGUEIREDO NETO, A. M.; BARBERO, G.. Electric response of cells of ferrofluids to ac external electric field: dependence on the concentration of magnetic particles and on the electrodes. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, v. 874, p. 8-pg., 2020-10-01. (14/50983-3, 16/24531-3)

TCHAKALOVA, VERA; OLIVEIRA, CRISTIANO LUIS PINTO; FIGUEIREDO NETO, ANTONIO MARTINS. New Lyotropic Complex Fluid Structured in Sheets of Ellipsoidal Micelles Solubilizing Fragrance Oils. ACS OMEGA, v. 8, n. 32, p. 17-pg., 2023-07-31. (14/50983-3, 16/24531-3)

LIMA, C. R. R. C.; LIMA, R. J. S.; BANDEIRA, A. C. C.; COUTO, R. A. A.; VELASCO, M. V. R.; BORDALLO, H. N.; OLIVEIRA, C. L. P.. Alterations promoted by acid straightening and/or bleaching in hair microstructures. JOURNAL OF APPLIED CRYSTALLOGRAPHY, v. 56, p. 13-pg., 2023-08-01. (11/50318-1, 16/24531-3)