Genomic insights of Klebsiella pneumoniae isolated from a native Amazonian fish reveal wide resistome against heavy metals, disinfectants, and clinically relevant antibiotics

Author(s)

Cerdeira, Louise ^{[1, 2, 3]} ; Monte, Daniel F. M. ^{[4, 2]} ; Fuga, Bruna ^{[1, 2]} ; Sellera, Fabio P. ^{[5, 2]} ; Neves, Ingrith ^{[1, 2]} ; Rodrigues, Larissa ^{[1, 2]} ; Landgraf, Mariza ^[4] ; Lincopan, Nilton ^{[6, 1, 2]}

Affiliation

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin Anal, Sao Paulo - Brazil

^[2] One Hlth Brazilian Resistance Project OneBR, Sao Paulo - Brazil

^[3] Monash Univ, Dept Infect Dis, Cent Clin Sch, Clayton, Vic - Australia

^[4] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, Food Res Ctr, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Internal Med, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo - Brazil

• Document type: Journal article
• Source: Genomics; v. 112, n. 6, p. 5143-5146, NOV 2020.
• Web of Science Citations: 0

Abstract

A multidrug-resistant CTX-M-15-producing Klebsiella pneumoniae (KpP1 strain) was isolated from a native Amazonian fish (Brachyplatystoma filamentosum) at the Brazilian Amazon. The strain was identified by MALDI-TOF. The genome was extracted, purified and a Nextera DNA Flex library was prepared and sequenced by Illumina platform. The sequenced genome was de novo assembled using Unicycler and in silico prediction accomplished by curated bioinformatics tools. The size of the genome is 5.6 Mb with 5715 genes. Whole-genome sequencing analysis revealed the presence of wide resistome, with genes conferring resistance to clinically relevant antibiotics, heavy metals and disinfectants. The KpP1 strain was assigned to the sequence type ST3827, KL111 (wzi113) and O3b locus. Native freshwater fish sold in wet markets of the Amazonian region could be an important vehicle for transmission of multidrug-resistant bacteria to humans. This study may give genomic insights on the spread of critical-priority WHO pathogens in a One Health context. (AU)

• FAPESP's process: 16/03044-7 - Genetic diversity, virulence genes and antimicrobial resistance of Salmonella spp. isolated from poultry slaughterhouse
• Grantee: Daniel Farias Marinho Do Monte
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/08593-9 - Pan-Resistome of beta-lactamase (KPC-2, CTX-M-8, CTX-M-15)-producing Klebsiella pneumoniae and Escherichia coli isolates endemic in Brazil
• Grantee: Nilton Erbet Lincopan Huenuman
• Support Opportunities: Regular Research Grants