Improvement of carotenoids production from Rhodotorula glutinis CCT-2186

Author(s)

Mussagy, Cassamo Ussemane ^{[1, 2]} ; Carizia Guimaraes, Ana Alice ^[2] ; Ferreira Rocha, Lara Vicente ^[2] ; Winterburn, James ^[3] ; Santos-Ebinuma, Valeria de Carvalho ^[2] ; Brandao Pereira, Jorge Fernando ^{[4, 2]}

Affiliation

^[1] Univ Aveiro, CICECO Aveiro Inst Mat, Dept Chem, P-3810193 Aveiro - Portugal

^[2] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Engn Bioproc & Biotechnol, BR-14800903 Araraquara, SP - Brazil

^[3] Univ Manchester, Dept Chem Engn & Analyt Sci, Manchester, Lancs - England

^[4] Univ Coimbra, Dept Chem Engn, CIEPQPF, Rua Silvio Lima, Polo 2 Pinhal Marrocos, P-3030790 Coimbra - Portugal

• Document type: Journal article
• Source: Biochemical Engineering Journal; v. 165, JAN 15 2021.
• Web of Science Citations: 5

Abstract

Rhodotorula strains can produce industrial valuable bioproducts. In this work, the production of carotenoids, and lipids by Rhodotorula glutinis using different nitrogen sources was evaluated. Two statistical experimental designs were applied to improve carotenoid production: a first 2(S-1) fractional factorial design evaluating the influence of independent variables pH, nitrogen source, glucose, KH2PO4 and MgSO4 concentration; a second 2(2) central factorial design to optimize the effect of pH and nitrogen sources. After the optimization using two statistical designs, a culture mediium composed of (in g/L) glucose (10), asparagine (10), NH4NO3 (4), KH2PO4 (0.52), MgSO 4 (0.52) was found as the best for the production of carotenoids at a pH 5 and 30 degrees C. The best bioprocess was scaled-up to a 5 L stirred-tank bioreactor. The change to a bioreactor allowed to improve aeration and agitation conditions, and consequently, increasing the production yields (m/v) in, approximately, 25.83 %, 11.88 %, 24.50 % and 10.32 % for beta-carotene, torularhodin, torulene and lipids, respectively. The combined supplementation of the culture media with both organic (asparagine) and inorganic nitrogen (ammonium nitrate) sources was primordial for enhancing the carotenogenesis. R. glutinis are very efficient in the production of valuable carotenoids and lipids, presenting high potential of yeast for the industrial production of more sustainable cosmetic, pharmaceutical, and food products. (AU)

• FAPESP's process: 17/50303-0 - Sustainable citrus waste biorefining as a source of value added bioproducts
• Grantee: Valéria de Carvalho Santos Ebinuma
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/16424-7 - Optimization and scale-up of liquid-liquid extraction process with ionic liquids (ILs) as a sustainable tool for the separation of the anti-leukemia biopharmaceutical L-asparaginase (ASPase)
• Grantee: Jorge Pereira
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 14/01580-3 - Biotechnological process for the development of new natural colorants from microorganisms for industrial application
• Grantee: Valéria de Carvalho Santos Ebinuma
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 14/19793-3 - Optimization and scale-up of novel Ionic-Liquid-based purification processes for recombinant green fluorescent protein produced by Escherichia coli "GFPurIL"
• Grantee: Sandro Roberto Valentini
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/09618-3 - Optimization of carotenoid extraction from yeast Rhodotorula glutinis CCT-2186 using biocompatible solvents
• Grantee: Lara Vicente Ferreira Rocha
• Support Opportunities: Scholarships in Brazil - Scientific Initiation