Bactericidal activity and biofilm inhibition of F18 bioactive glass against Staphylococcus aureus

Author(s)

Passos, Tathiane Ferroni ^{[1, 2]} ; Souza, Marina Trevelin ^[3] ; Zanotto, Edgar Dutra ^[3] ; de Souza, Clovis Wesley Oliveira ^{[1, 2]}

Affiliation

^[1] Univ Fed Sao Carlos, Biotechnol Grad Program PPGBiotec UFSCar, Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Microbiol & Parasitol Lab LMP, Dept Morphol & Pathol, Sao Carlos, SP - Brazil

^[3] Univ Fed Sao Carlos, Dept Mat Engn, Vitreous Mat Lab LaMaV, Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Materials Science & Engineering C-Materials for Biological Applications; v. 118, JAN 2021.
• Web of Science Citations: 1

Abstract

Antimicrobial treatment failure has been increasing at alarming rates. In this context, the bactericidal properties of biocompatible antimicrobial agents have been widely studied. F18 is a recently developed bioactive glass that presents a much wider working range when compared to other bioactive glasses, a feature that allows it to be used for coating metallic implants, sintering scaffolds or manufacturing fibers for wound healing applications. The aim of this study was to investigate the in vitro bactericidal and anti-biofilm activity of F18 glass as a powder and as a coating on steel samples, and to explore the effects of its dissolution products at concentrations from 3 mg/mL to 50 mg/mL against the Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) biofilms. Furthermore, we intend to verify whether changes in the medium pH could influence the bactericidal activity of F18. The results indicated that F18 presented bactericidal activity in preformed S. aureus and MRSA biofilms, reducing more than 6 logs of the viable cells that remained in contact with 50 mg/mL for 24 h. Moreover, an anti-biofilm activity was observed after 12 h of direct contact, with a drop of more than 6 logs of the viable bacterial population. Neutralization of the F18 solution pH decreased its bactericidal efficacy. These results indicate that the F18 glass could be considered as an alternative material for controlling and treating infections by S. aureus. (AU)

• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 13/07793-6 - CEPIV - Center for Teaching, Research and Innovation in Glass
• Grantee: Edgar Dutra Zanotto
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC