| Full text | |
| Author(s): |
Teixeira-Silva, Natalia Sousa
[1, 2, 3]
;
Carvalho Schaker, Patricia Dayane
[1]
;
Silva Rody, Hugo Vianna
[1]
;
Maia, Thiago
[1]
;
Garner, Christopher M.
[2, 4, 3]
;
Gassmann, Walter
[2, 4, 3]
;
Monteiro-Vitorello, Claudia Barros
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, BR-13418900 Piracicaba - Brazil
[2] Univ Missouri, Interdisciplinary Plant Grp, Columbia, MO 65211 - USA
[3] Univ Missouri, Christopher S Bond Life Sci Ctr, Columbia, MO 65211 - USA
[4] Univ Missouri, Coll Agr Food & Nat Resources, Div Plant Sci, Columbia, MO 65211 - USA
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | JOURNAL OF FUNGI; v. 6, n. 4 DEC 2020. |
| Web of Science Citations: | 1 |
| Abstract | |
Sporisorium scitamineum is a biotrophic fungus causing sugarcane smut disease. In this study, we set up a pipeline and used genomic and dual transcriptomic data previously obtained by our group to identify candidate effectors of S. scitamineum and their expression profiles in infected smut-resistant and susceptible sugarcane plants. The expression profile of different genes after infection in contrasting sugarcane genotypes assessed by RT-qPCR depended on the plant genotypes and disease progression. Three candidate effector genes expressed earlier only in resistant plants, four expressed in both genotypes, and three later in susceptible plants. Ten genes were cloned and transiently expressed in N. benthamiana leaves to determine their subcellular location, while four localized in more than one compartment. Two candidates, g3890 having a nucleoplasmic and mitochondrial location and g5159 targeting the plant cell wall, were selected to obtain their possible corresponding host targets using co-immunoprecipitation (CoIP) experiments and mass spectrometry. Various potential interactors were identified, including subunits of the protein phosphatase 2A and an endochitinase. We investigated the presence of orthologs in sugarcane and using transcriptome data present their expression profiles. Orthologs of sugarcane shared around 70% similarity. Identifying a set of putative fungal effectors and their plant targets provides a valuable resource for functional characterization of the molecular events leading to smut resistance in sugarcane plants and uncovers further opportunities for investigation. (AU) | |
| FAPESP's process: | 17/13268-2 - Genomic and functional studies of the interaction Sporisorium scitamineum-cana and other smut fungi: advances and challenges |
| Grantee: | Claudia Barros Monteiro Vitorello |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/04555-0 - RGAs sugarcane expression profile during infection with various pathogens |
| Grantee: | Hugo Rody Vianna Silva |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 16/04429-0 - Plant cell localization of candidate effector proteins from Sporisorium scitamineum, the sugarcane smut pathogen |
| Grantee: | Patricia Dayane Carvalho Schaker |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 16/16376-8 - Functional analysis of candidate effector proteins during Sporisorium scitamineum x sugarcane interaction |
| Grantee: | Natália de Sousa Teixeira e Silva |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 17/25477-5 - Identification of candidate interactors and global response pathways activated by Sporisorium scitamineum effectors |
| Grantee: | Natália de Sousa Teixeira e Silva |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 16/17545-8 - Genes, genomes & transposable elements contribution to plant-microbe interaction: a sugarcane study case |
| Grantee: | Marie-Anne Van Sluys |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Thematic Grants |
| FAPESP's process: | 13/25599-2 - Study of sugarcane metabolism modulation by the plant pathogenic fungus Sporisorium scitamineum |
| Grantee: | Patricia Dayane Carvalho Schaker |
| Support Opportunities: | Scholarships in Brazil - Doctorate |