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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Intradermal Delivery of Dendritic Cell-Targeting Chimeric mAbs Genetically Fused to Type 2 Dengue Virus Nonstructural Protein 1

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Author(s):
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Pereira, Lennon Ramos [1] ; Vicentin, Elaine Cristina Matos [2] ; Pereira, Sara Araujo [1] ; Maeda, Denicar Lina Nascimento Fabris [1] ; Alves, Rubens Prince dos Santos [1] ; Andreata-Santos, Robert [1] ; Sousa, Francielle Tramontini Gomes de [3] ; Yamamoto, Marcio Massao [2] ; Castro-Amarante, Maria Fernanda [1] ; Favaro, Marianna Teixeira de Pinho [1] ; Romano, Camila Malta [3] ; Sabino, Ester Cerdeira [3] ; Boscardin, Silvia Beatriz [2] ; Ferreira, Luis Carlos de Souza [1]
Total Authors: 14
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, BR-05508000 Sao Paulo - Brazil
[3] Univ Sao Paulo, Clin Hosp HCFMUSP, Fac Med, BR-05403000 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: VACCINES; v. 8, n. 4 DEC 2020.
Web of Science Citations: 0
Abstract

Targeting dendritic cells (DCs) by means of monoclonal antibodies (mAbs) capable of binding their surface receptors (DEC205 and DCIR2) has previously been shown to enhance the immunogenicity of genetically fused antigens. This approach has been repeatedly demonstrated to enhance the induced immune responses to passenger antigens and thus represents a promising therapeutic and/or prophylactic strategy against different infectious diseases. Additionally, under experimental conditions, chimeric alpha DEC205 or alpha DCIR2 mAbs are usually administered via an intraperitoneal (i.p.) route, which is not reproducible in clinical settings. In this study, we characterized the delivery of chimeric alpha DEC205 or alpha DCIR2 mAbs via an intradermal (i.d.) route, compared the elicited humoral immune responses, and evaluated the safety of this potential immunization strategy under preclinical conditions. As a model antigen, we used type 2 dengue virus (DENV2) nonstructural protein 1 (NS1). The results show that the administration of chimeric DC-targeting mAbs via the i.d. route induced humoral immune responses to the passenger antigen equivalent or superior to those elicited by i.p. immunization with no toxic effects to the animals. Collectively, these results clearly indicate that i.d. administration of DC-targeting chimeric mAbs presents promising approaches for the development of subunit vaccines, particularly against DENV and other flaviviruses. (AU)

FAPESP's process: 13/26942-2 - Development of a vaccine formulation against dengue based on the nonstructural protein 1 (NS1) administered by the intradermal route
Grantee:Lennon Ramos Pereira
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 14/04303-0 - Identification of epitopes present in the e protein of dengue virus type 2 (DENV2) capable of generating neutralizing antibodies without causing exacerbation of viral replication
Grantee:Denicar Lina Nascimento Fabris
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 16/05570-8 - Use of Zika virus recombinant proteins for development of diagnostic methods and vaccines
Grantee:Lennon Ramos Pereira
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 13/01702-9 - Evaluation of endothelial permeability to study dengue pathogenesis and screen compounds with therapeutic potential
Grantee:Francielle Tramontini Gomes de Sousa
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/11442-4 - Study of protective mechanisms induced by vaccination of mice with fusion antibodies that target the dengue virus proteins to dendritic cells
Grantee:Silvia Beatriz Boscardin
Support Opportunities: Regular Research Grants
FAPESP's process: 14/17595-0 - New vaccine research based on recombinant proteins of dengue virus
Grantee:Luis Carlos de Souza Ferreira
Support Opportunities: Regular Research Grants
FAPESP's process: 13/01690-0 - Establishment of an in vitro model of vascular permeability evaluation to study the hemorrhagic dengue fever pathogenesis and screening of compounds with therapeutic potential
Grantee:Ester Cerdeira Sabino
Support Opportunities: Regular Research Grants