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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

CheckV assesses the quality and completeness of metagenome-assembled viral genomes

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Author(s):
Nayfach, Stephen [1] ; Camargo, Antonio Pedro [2] ; Schulz, Frederik [1] ; Eloe-Fadrosh, Emiley [1] ; Roux, Simon [1] ; Kyrpides, Nikos C. [1]
Total Authors: 6
Affiliation:
[1] US DOE, Joint Genome Inst, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 - USA
[2] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut Microbiol & Immunol, Campinas - Brazil
Total Affiliations: 2
Document type: Journal article
Source: NATURE BIOTECHNOLOGY; v. 39, n. 5 DEC 2020.
Web of Science Citations: 8
Abstract

Millions of new viral sequences have been identified from metagenomes, but the quality and completeness of these sequences vary considerably. Here we present CheckV, an automated pipeline for identifying closed viral genomes, estimating the completeness of genome fragments and removing flanking host regions from integrated proviruses. CheckV estimates completeness by comparing sequences with a large database of complete viral genomes, including 76,262 identified from a systematic search of publicly available metagenomes, metatranscriptomes and metaviromes. After validation on mock datasets and comparison to existing methods, we applied CheckV to large and diverse collections of metagenome-assembled viral sequences, including IMG/VR and the Global Ocean Virome. This revealed 44,652 high-quality viral genomes (that is, >90% complete), although the vast majority of sequences were small fragments, which highlights the challenge of assembling viral genomes from short-read metagenomes. Additionally, we found that removal of host contamination substantially improved the accurate identification of auxiliary metabolic genes and interpretation of viral-encoded functions. The quality of viral genomes assembled from metagenome data is assessed by CheckV. (AU)

FAPESP's process: 16/23218-0 - The Genomics for Climate Change Research Center
Grantee:Edi Lúcia Sartorato
Support Opportunities: Research Grants - Research Centers in Engineering Program
FAPESP's process: 18/04240-0 - Metagenomic investigation of the microbiomes of plants adapted to phosphorus nutritional limitation
Grantee:Antônio Pedro de Castello Branco da Rocha Camargo
Support Opportunities: Scholarships in Brazil - Doctorate