Paternal exercise protects against liver steatosis in the male offspring of mice submitted to high fat diet

Author(s):Show less -

Batista, Rogerio Oliveira ^{[1, 2]} ; Budu, Alexandre ^[1] ; Alves-Silva, Thais ^[1] ; Arakaki, Aline Midori ^{[1, 2]} ; Gregnani, Marcos Fernandes S. ^[1] ; Rodrigues Hungaro, Talita G. ^{[1, 2]} ; Burgos-Silva, Marina ^[3] ; Wasinski, Frederick ^{[1, 2]} ; Lanzoni, Valeria Pereira ^[4] ; Saraiva Camara, Niels Olsen ^[3] ; Oyama, Lila Missae ^[5] ; Bader, Michael ^{[6, 7, 8, 9, 10]} ; Araujo, Ronaldo Carvalho ^{[1, 2]}

Affiliation

^[1] Univ Fed Sao Paulo, Dept Biophys, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Med, Nephrol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Immunol, Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Dept Pathol, Sao Paulo - Brazil

^[5] Univ Fed Sao Paulo, Dept Physiol, Sao Paulo - Brazil

^[6] Max Delbruck Ctr Mol Med, Berlin - Germany

^[7] Univ Lubeck, Inst Biol, Lubeck - Germany

^[8] Charite, Berlin - Germany

^[9] DZHK German Ctr Cardiovasc Res, Partner Site Berlin, Berlin - Germany

^[10] Berlin Inst Hlth BIH, Berlin - Germany

• Document type: Journal article
• Source: Life Sciences; v. 263, DEC 15 2020.
• Web of Science Citations: 0

Abstract

Parental lifestyle has been related to alterations in the phenotype of their offspring. Obese sires can induce offspring insulin resistance as well as increase susceptibility to obesity. On the other hand, obese sires submitted to voluntary exercise ameliorate the deleterious metabolic effects on their offspring. However, there are no studies reporting the effect of programmed exercise training of lean sires on offspring metabolism. Aims: This study aimed to investigate the role of swimming training of sires for 6 weeks on the offspring metabolic phenotype. Main methods: Male C57BL/6 mice fed a control diet were divided into sedentary and swimming groups. After the exercise, they were mated with sedentary females, and body weight and molecular parameters of the offspring were subsequently monitored. Key findings: Swimming decreased the gene expression of Fasn and Acaca in the testes and increased the AMPK protein content in the testes and epididymis of the sires. The progeny presented a low weight at P1, which reached a normal level at P60 and at P90 the animals were challenged with HFD for 16 weeks. The male offspring of trained sires presented less body weight gain than the control group. The level of steatosis decreased in the male offspring from trained sires. The gene expression of Prkaa2, Ppar-1 alpha and Cpt-1 was also increased in the liver of male offspring from trained sires. Significance: Taken together, these findings suggest that paternal exercise training can improve the metabolic profile in the liver of the progeny, thereby ameliorating the effects of obesity. (AU)

• FAPESP's process: 15/20082-7 - Kallikrein kinin system in physical exercise and metabolism
• Grantee: Ronaldo de Carvalho Araújo
• Support Opportunities: Research Projects - Thematic Grants