Multivariate optimization of low-temperature cleanup followed by dispersive solid-phase extraction for detection of Bisphenol A and benzophenones in infant formula

Author(s)

Galindo, Marcella Vitoria ^[1] ; Oliveira, Wellington da Silva ^[1] ; Godoy, Helena Teixeira ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Food Engn, Dept Food Sci, BR-13083862 Campinas, SP - Brazil

• Document type: Journal article
• Source: Journal of Chromatography A; v. 1635, JAN 4 2021.
• Web of Science Citations: 1

Abstract

A simple and effective analytical method to determine six contaminants, including five benzophenones (BP, BP-1, BP-3, BP-8, and BP-12) and bisphenol A (BPA) in infant formulas was developed in this study. For this, a sequential experimental design was used to optimize the extraction and cleanup method using low temperature partition (LTP) combined with dispersive solid phase extraction (dSPE). The effect of primary secondary amine (PSA), sodium chloride (NaCl), graphitized carbon black (GCB), octadecyl (C18), strong anion exchanger (SAX), water, acetonitrile (ACN) and, ultrasound (US) time were evaluated using a sequential design of experiments including a Plackett-Burman, a central composite rotatable design, and the Derringer and Suich's tool. The method was validated, and it showed a limit of quantification varying from 0.06 to 2 mg.kg(-1), good precision (< 20% RSD), and recovery (52-106%). The method proposed was applied to twenty-five samples of commercial infant formulas. (C) 2020 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 17/11635-8 - Residues and contaminants in baby food and baby bottles: development and validation of analytical methods
• Grantee: Helena Teixeira Godoy
• Support Opportunities: Regular Research Grants