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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Airways exposure of bacterial superantigen SEB enhances bone marrow eosinophil population and facilitates its egress to blood and lung tissue

Full text
Author(s):
Pinheiro-Torres, A. S. [1] ; Ferreira-Duarte, A. P. [1] ; Takeshita, W. M. [1] ; Gushiken, V. O. [1] ; Roncalho-Buck, I. A. [1] ; Anhe, G. F. [2] ; Antunes, E. [2] ; DeSouza, I. A. [1]
Total Authors: 8
Affiliation:
[1] Fac Med Jundiai FMJ, Dept Biol & Physiol, BR-13202550 Jundiai, SP - Brazil
[2] Univ Campinas UNICAMP, Fac Med Sci, Dept Pharmacol, Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Life Sciences; v. 264, JAN 1 2021.
Web of Science Citations: 0
Abstract

Background: Differentiation of bone marrow eosinophils (BM-EO) and its trafficking to peripheral blood and respiratory mucosa are a hallmark of inflammatory diseases. Staphylococcal enterotoxin B (SEB) has been shown to aggravate airways eosinophilic inflammation. This study aimed to investigate the effects of mouse airways SEB exposure on BM-EO population, as well as its adhesive properties and release of cytokines/chemokines that orchestrate BM-EO trafficking to lungs. Methods: Male BALB/c mice were intranasally exposed to SEB (1 mu g), and at 4, 16, 24 and 48 h thereafter, bone marrow (BM), circulating blood and bronchoalveolar lavage (BAL) fluid were collected. Levels of cytokines/chemokines and expressions of VLA-4 and CCR3 in BM were evaluated. Adhesion of BM to ICAM-1 and VCAM-1 were also evaluated. Results: SEB exposure promoted a marked eosinophil influx to BAL at 16 and 24 h after exposure, which was accompanied by significant increases in counts of immature (16 h) and mature (4 to 48 h) forms of eosinophil in BM, along with blood eosinophilia (16 h). In BM, higher levels of eotaxin, IL-5, IL-4, IL-3 and IL-7 were detected at 16 to 48 h. SEB also significantly increased CCR3 expression and calcium levels in BM-EO, and enhanced the cell adhesion to ICAM-1 (24 h) and ICAM-1 (48 h). Conclusion: Airways SEB exposure increases the number of eosinophils in BM by mechanisms involving a network of cytokine and chemokine release, facilitating the BM-EO adhesion to ICAM-1 and VCAM-1 to gain access to the peripheral blood and lung tissues. (AU)

FAPESP's process: 17/15175-1 - Modulation of soluble guanylate cyclase and the intracellular levels of cyclic nucleotides in the lower urinary tract and prostate
Grantee:Edson Antunes
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 17/25867-8 - Effect of staphylococcal enterotoxins type A (SEA) and B (SEB) on the neutrophil dysfunction during Staphylococcus aureus-induced sepsis
Grantee:Ivani Aparecida de Souza
Support Opportunities: Regular Research Grants