Amino Acid and Carbohydrate Metabolism Are Coordinated to Maintain Energetic Balance during Drought in Sugarcane

Author(s):Show less -

Diniz, Augusto Lima ^[1] ; Rodrigues da Silva, Danielle Izilda ^{[2, 1, 3]} ; Lembke, Carolina Gimiliani ^[1] ; Lamas Costa, Maximiller Dal-Bianco ^{[1, 4]} ; ten-Caten, Felipe ^[1] ; Li, Forrest ^[5] ; Vilela, Romel Duarte ^[6] ; Menossi, Marcelo ^[7] ; Ware, Doreen ^{[5, 8]} ; Endres, Lauricio ^[6] ; Souza, Glaucia Mendes ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, BR-13418900 Piracicaba, SP - Brazil

^[3] Ohio State Univ, Ctr Appl Plant Sci CAPS, Columbus, OH 43210 - USA

^[4] Univ Fed Vicosa, Dept Bioquim & Biol Mol, BR-36570900 Vicosa, MG - Brazil

^[5] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 - USA

^[6] Univ Fed Alagoas, Ctr Ciencias Agr, BR-57100000 Rio Largo, AL - Brazil

^[7] Univ Estadual Campinas, Inst Biol, BR-13083862 Campinas, SP - Brazil

^[8] USDA ARS, NAA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 - USA

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 21, n. 23 DEC 2020.
• Web of Science Citations: 0

Abstract

The ability to expand crop plantations without irrigation is a major goal to increase agriculture sustainability. To achieve this end, we need to understand the mechanisms that govern plant growth responses under drought conditions. In this study, we combined physiological, transcriptomic, and genomic data to provide a comprehensive picture of drought and recovery responses in the leaves and roots of sugarcane. Transcriptomic profiling using oligoarrays and RNA-seq identified 2898 (out of 21,902) and 46,062 (out of 373,869) transcripts as differentially expressed, respectively. Co-expression analysis revealed modules enriched in photosynthesis, small molecule metabolism, alpha-amino acid metabolism, trehalose biosynthesis, serine family amino acid metabolism, and carbohydrate transport. Together, our findings reveal that carbohydrate metabolism is coordinated with the degradation of amino acids to provide carbon skeletons to the tricarboxylic acid cycle. This coordination may help to maintain energetic balance during drought stress adaptation, facilitating recovery after the stress is alleviated. Our results shed light on candidate regulatory elements and pave the way to biotechnology strategies towards the development of drought-tolerant sugarcane plants. (AU)

• FAPESP's process: 13/13659-0 - Identification of sugarcane gene networks and active genome regions associated to drought
• Grantee: Danielle Izilda Rodrigues da Silva
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/01764-0 - Comprehensive analysis of global sugarcane transcriptome changes in response to drought
• Grantee: Danielle Izilda Rodrigues da Silva
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 17/02270-6 - Evaluation of contrasting progenies transcriptome for the accumulation of biomass and identification of regulation networks
• Grantee: Augusto Lima Diniz
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/50921-8 - Signaling and regulatory network studies associated to the energy cane
• Grantee: Glaucia Mendes Souza
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 19/15852-9 - The transcriptional landscape and co-expression networks of sugarcane
• Grantee: Augusto Lima Diniz
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor