Reproductive performance of lambari (Astyanax altiparanae) in a seminatural system using different protocols

Author(s)

Roza de Abreu, Mariana ^[1] ; Silva, Laiza Maria de Jesus ^[1] ; Figueiredo-Ariki, Daniel Guimaraes ^{[1, 2]} ; Sato, Rafael Tomoda ^[1] ; Kuradomi, Rafael Yutaka ^{[1, 3]} ; Batlouni, Sergio Ricardo ^[1]

Affiliation

^[1] Univ Estadual Paulista UNESP, Ctr Aquicultura, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil

^[2] Embrapa Amazonia Ocidental, Rodovia AM-010, Km 29 S-N, Zona Rural, BR-69010970 Manaus, Amazonas - Brazil

^[3] Univ Fed Amazonas UFAM, ICET, Itacoatiara, Amazonas - Brazil

• Document type: Journal article
• Source: AQUACULTURE RESEARCH; v. 52, n. 2, p. 471-483, FEB 2021.
• Web of Science Citations: 0

Abstract

We evaluated lambari (Astyanax altiparanae) reproductive performance by administration of gradually increasing doses of analogue of salmon gonadotropin-releasing hormone (sGnRHa). We performed two consecutive experiments applying six sGnRHa single doses in three replicates (ranging from 1 to 160 mu g/kg) (Exp. 1) and three sGnRHa-fractioned doses in four replicates (10% + 90% - 12 hr interval) (10, 100 and 1,000 mu g/kg) (Exp. 2) in 6 months old breeders. In both experiments, 6 mg of Carp pituitary extract (CPE)/kg and saline solution (without hormonal therapy) were used as positive and negative controls, respectively. Reproductive performance was recorded for both experiments; and quantification of 17 alpha-20 beta-dihydroxy-4-pregnen-3-one (17,20 beta-P) and prostaglandin F(2 alpha)plasma levels and ovarian histological evaluations were introduced in Exp. 2. Using a single dose, the spawning rate was low and similar between all groups (from 0% to 20%). Using fractioned doses, the spawning rate of CPE (60%) was higher than other groups (from 10% to 25%), but similar to 1,000 mu g of sGnRHa/kg (40%). 17,20 beta-P increased at the final sampling regardless of treatment (including controls) or successful spawning, suggesting that the simple grouping of fish caused this increase. 17,20 beta-P and PGF(2 alpha)plasma levels did not explain ovulation failures in this species. CPE-fractioned doses improved lambari ovulation and provided final maturation in all evaluated females; however, hormonal induction was not conditional for obtaining ovulation since spawning was registered in negative control. Concluding, CPE-fractioned doses protocol can be applied to increase reproductive performance of this species. (AU)

• FAPESP's process: 16/25988-7 - Induction to final maturation and ovulation of lambari (Astyanax altiparanae) with salmon GnRH analogue
• Grantee: Mariana Roza de Abreu
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/05741-5 - Gene expression profile of LHb in the pituitary of Astyanax altiparanae females, induced with synthetic hormones, using real-time quantitative polymerase chain reaction (RT-qPCR)
• Grantee: Mariana Roza de Abreu
• Support type: Scholarships abroad - Research Internship - Doctorate