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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Reproductive performance of lambari (Astyanax altiparanae) in a seminatural system using different protocols

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Author(s):
Roza de Abreu, Mariana [1] ; Silva, Laiza Maria de Jesus [1] ; Figueiredo-Ariki, Daniel Guimaraes [1, 2] ; Sato, Rafael Tomoda [1] ; Kuradomi, Rafael Yutaka [1, 3] ; Batlouni, Sergio Ricardo [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Paulista UNESP, Ctr Aquicultura, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil
[2] Embrapa Amazonia Ocidental, Rodovia AM-010, Km 29 S-N, Zona Rural, BR-69010970 Manaus, Amazonas - Brazil
[3] Univ Fed Amazonas UFAM, ICET, Itacoatiara, Amazonas - Brazil
Total Affiliations: 3
Document type: Journal article
Source: AQUACULTURE RESEARCH; v. 52, n. 2, p. 471-483, FEB 2021.
Web of Science Citations: 0
Abstract

We evaluated lambari (Astyanax altiparanae) reproductive performance by administration of gradually increasing doses of analogue of salmon gonadotropin-releasing hormone (sGnRHa). We performed two consecutive experiments applying six sGnRHa single doses in three replicates (ranging from 1 to 160 mu g/kg) (Exp. 1) and three sGnRHa-fractioned doses in four replicates (10% + 90% - 12 hr interval) (10, 100 and 1,000 mu g/kg) (Exp. 2) in 6 months old breeders. In both experiments, 6 mg of Carp pituitary extract (CPE)/kg and saline solution (without hormonal therapy) were used as positive and negative controls, respectively. Reproductive performance was recorded for both experiments; and quantification of 17 alpha-20 beta-dihydroxy-4-pregnen-3-one (17,20 beta-P) and prostaglandin F(2 alpha)plasma levels and ovarian histological evaluations were introduced in Exp. 2. Using a single dose, the spawning rate was low and similar between all groups (from 0% to 20%). Using fractioned doses, the spawning rate of CPE (60%) was higher than other groups (from 10% to 25%), but similar to 1,000 mu g of sGnRHa/kg (40%). 17,20 beta-P increased at the final sampling regardless of treatment (including controls) or successful spawning, suggesting that the simple grouping of fish caused this increase. 17,20 beta-P and PGF(2 alpha)plasma levels did not explain ovulation failures in this species. CPE-fractioned doses improved lambari ovulation and provided final maturation in all evaluated females; however, hormonal induction was not conditional for obtaining ovulation since spawning was registered in negative control. Concluding, CPE-fractioned doses protocol can be applied to increase reproductive performance of this species. (AU)

FAPESP's process: 16/25988-7 - Induction to final maturation and ovulation of lambari (Astyanax altiparanae) with salmon GnRH analogue
Grantee:Mariana Roza de Abreu
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 19/05741-5 - Gene expression profile of LHb in the pituitary of Astyanax altiparanae females, induced with synthetic hormones, using real-time quantitative polymerase chain reaction (RT-qPCR)
Grantee:Mariana Roza de Abreu
Support type: Scholarships abroad - Research Internship - Doctorate