In aquaculture the use of sterile animals is advantageous, as it may facilitate increased growth and reduce the impact of escaped animals. In fish, triploidization is one of the most commonly employed techniques in large sclae. The yellowtail tetra Astyanax altiparanae is a native species of importance to aquaculture, due to its small size, resistance to suboptimal environmental conditions and ease of reproduction. Previously studies showed the viability of triploidization protocol and also, showed that female triploid are sterile and present increased growth. However, studies are necessary for the viability of the production of 100% triploid female, using tetraploid males, which, will facilitated the management and guarantee an offspring 100% triploid; with such studies, important technologies like the gynogenesis will also be available, a technique that generate progeny with maternal inheritance that will possibility to elucidate the genetic determination system in lambari (XY or ZW) and guarantee offsprings 100% females. Therefore, the aim of this project is study the application of tetraploidization and gynogenesis protocols, which together, will possibility the large sacle production of 100% triploid females. Additionally, both protocols will open new possibilities of future studies in the area of biotechnology applied to aquaculture.
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