Chromosome set manipulation techniques on lambari (Astyanax altiparanae)

• Grant number: 16/12383-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2016
• End date: November 30, 2018
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Laura Satiko Okada Nakaghi
• Grantee: Nivaldo Ferreira do Nascimento
• Host Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated research grant: 10/17429-1 - Chimerism in native fish species as a tool for surrogate propagation in aquaculture, AP.JP

Abstract

In aquaculture the use of sterile animals is advantageous, as it may facilitate increased growth and reduce the impact of escaped animals. In fish, triploidization is one of the most commonly employed techniques in large sclae. The yellowtail tetra Astyanax altiparanae is a native species of importance to aquaculture, due to its small size, resistance to suboptimal environmental conditions and ease of reproduction. Previously studies showed the viability of triploidization protocol and also, showed that female triploid are sterile and present increased growth. However, studies are necessary for the viability of the production of 100% triploid female, using tetraploid males, which, will facilitated the management and guarantee an offspring 100% triploid; with such studies, important technologies like the gynogenesis will also be available, a technique that generate progeny with maternal inheritance that will possibility to elucidate the genetic determination system in lambari (XY or ZW) and guarantee offsprings 100% females. Therefore, the aim of this project is study the application of tetraploidization and gynogenesis protocols, which together, will possibility the large sacle production of 100% triploid females. Additionally, both protocols will open new possibilities of future studies in the area of biotechnology applied to aquaculture.

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DO NASCIMENTO, NIVALDO FERREIRA; MONZANI, PAULO SERGIO; PEREIRA-SANTOS, MATHEUS; NIEDZIELSKI, DAIANE; SENHORINI, JOSE AUGUSTO; SILVA, LUCIANO ANDRADE; NAKAGHI, LAURA SATIKO OKADA; YASUI, GEORGE SHIGUEKI. The first case of induced gynogenesis in Neotropical fishes using the yellowtail tetra (Astyanax altiparanae) as a model organism. Aquaculture, v. 514, JAN 1 2020. (10/17429-1, 16/12383-0, 11/11664-1)

MARTINS, LEANDRO FREITAS; HILBIG, CLEONICE CRISTINA; YASUI, GEORGE SHIGUEKI; MONZANI, PAULO SERGIO; SENHORINI, JOSE AUGUSTO; OKADA NAKAGHI, LAURA SATIKO; DO NASCIMENTO, NIVALDO FERREIRA. Return temperature after heat shock affects the production of tetraploids in the yellowtail tetra Astyanax altiparanae. ZYGOTE, v. 29, n. 1, p. 82-86, FEB 2021. (16/12383-0, 11/11664-1, 10/17429-1)

LAZARO, TALITA MARIA; ROCHA, NATHALIA RAISSA DE ALCANTARA; MONZANI, PAULO SERGIO; NAKAGHI, LAURA SATIKO OKADA; SENHORINI, JOSE AUGUSTO; YASUI, GEORGE SHIGUEKI; DO NASCIMENTO, NIVALDO FERREIRA. Sperm quality of spontaneously occurring gynogenetic males in the yellowtail tetra Astyanax altiparanae. Journal of Applied Ichthyology, v. 37, n. 6, JUL 2021. (11/11664-1, 16/16386-3, 16/12383-0, 10/17429-1)

DO NASCIMENTO, NIVALDO FERREIRA; LAZARO, TALITA MARIA; DE ALCANTARA, NATHALIA RAISSA; ROCHA; SENHORINI, JOSE AUGUSTO; DOS SANTOS, SILVIO CARLOS ALVES; NAKAGHI, LAURA SATIKO OKADA; YASUI, GEORGE SHIGUEKI. In vivo storage of oocytes leads to lower survival, increased abnormalities and may affect the ploidy status in the yellowtail tetra Astyanax altiparanae. ZYGOTE, v. 26, n. 6, p. 471-475, DEC 2018. (10/17429-1, 11/11664-1, 16/12383-0)

N. F. NASCIMENTO; A. P. SANTOS-SILVA; M. PEREIRA-SANTOS; N. LEVY-PEREIRA; J. A. SENHORINI; G. S. YASUI. Manual vs. automated erythrocyte assessment for polyploidy detection in neotropical fish: a comparative study. Brazilian Journal of Biology, v. 84, 2024-05-10. (16/12383-0, 10/17429-1, 11/11664-1)

DO NASCIMENTO, NIVALDO FERREIRA; PEREIRA-SANTOS, MATHEUS; LEVY-PEREIRA, NYCOLAS; MONZANI, PAULO SERGIO; NIEDZIELSKI, DAIANE; FUJIMOTO, TAKAFUMI; SENHORINI, JOSE AUGUSTO; OKADA NAKAGHI, LAURA SATIKO; YASUI, GEORGE SHIGUEKI. High percentages of larval tetraploids in the yellowtail tetra Astyanax altiparanae induced by heat-shock: The first case in Neotropical characins. Aquaculture, v. 520, APR 15 2020. (10/17429-1, 16/12383-0, 11/11664-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

NASCIMENTO, Nivaldo Ferreira Do. Técnicas de manipulação cromossômica no lambari (Astyanax altiparanae). 2018. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.