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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

On the Challenges for the Diagnosis of SARS-CoV-2 Based on a Review of Current Methodologies

Full text
Author(s):
Mattioli, Isabela A. [1] ; Hassan, Ayaz [1] ; Oliveira Jr, Osvaldo N. ; Crespilho, Frank N. [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Sao Carlos Inst Chem, BR-13560970 Sao Carlos, SP - Brazil
Total Affiliations: 1
Document type: Review article
Source: ACS SENSORS; v. 5, n. 12, p. 3655-3677, DEC 24 2020.
Web of Science Citations: 1
Abstract

Diagnosis of COVID-19 has been challenging owing to the need for mass testing and for combining distinct types of detection to cover the different stages of the infection. In this review, we have surveyed the most used methodologies for diagnosis of COVID-19, which can be basically categorized into genetic-material detection and immunoassays. Detection of genetic material with real-time polymerase chain reaction (RT-PCR) and similar techniques has been achieved with high accuracy, but these methods are expensive and require time-consuming protocols which are not widely available, especially in less developed countries. Immunoassays for detecting a few antibodies, on the other hand, have been used for rapid, less expensive tests, but their accuracy in diagnosing infected individuals has been limited. We have therefore discussed the strengths and limitations of all of these methodologies, particularly in light of the required combination of tests owing to the long incubation periods. We identified the bottlenecks that prevented mass testing in many countries, and proposed strategies for further action, which are mostly associated with materials science and chemistry. Of special relevance are the methodologies which can be integrated into point-of-care (POC) devices and the use of artificial intelligence that do not require products from a well-developed biotech industry. (AU)

FAPESP's process: 19/15333-1 - Bio-photo-electrochemical hybrid cells for solar energy conversion
Grantee:Frank Nelson Crespilho
Support type: Regular Research Grants
FAPESP's process: 18/11071-0 - Development and application of disposable screen-printed composite electrodes based on metallic nanoparticles and graphite-polyurethane
Grantee:Isabela Alteia Mattioli
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 19/12053-8 - High performance electrodes applied in organic batteries and in biofuel cell
Grantee:Frank Nelson Crespilho
Support type: Regular Research Grants
FAPESP's process: 18/22214-6 - Toward a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
Grantee:Osvaldo Novais de Oliveira Junior
Support type: Research Projects - Thematic Grants