Design of experiments driven optimization of alkaline pretreatment and saccharification for sugarcane bagasse

Author(s)

Mota, Thatiane R. ^[1] ; Oliveira, Dyoni M. ^[1] ; Simister, Rachael ^[2] ; Whitehead, Caragh ^[2] ; Lanot, Alexandra ^[2] ; dos Santos, Wanderley D. ^[1] ; Rezende, Camila A. ^[3] ; McQueen-Mason, Simon J. ^[2] ; Gomez, Leonardo D. ^[2]

Affiliation

^[1] Univ Estadual Maringa, Dept Biochem, UEM, BR-87020900 Maringa, Parana - Brazil

^[2] Univ York, Dept Biol, Ctr Novel Agr Prod, CNAP, York YO10 5DD, N Yorkshire - England

^[3] Univ Estadual Campinas, Inst Chem, UNICAMP, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: Bioresource Technology; v. 321, FEB 2021.
• Web of Science Citations: 1

Abstract

To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 degrees C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 mu L). A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 degrees C for 40 min, with 12.5% of enzyme loading). Biomass compositional analyses showed that the pretreatments strongly removed lignin (up to 70%), silica (up to 80%) and promoted cellulose enrichment (25-110%). This robust design of experiments resulted in maximizing enzymatic hydrolysis efficiency of sugarcane bagasse and further indicated that this combined approach is versatile for other lignocellulosic biomasses. (AU)

• FAPESP's process: 18/23769-1 - Preparation and applications of nanostructures from plant biomass
• Grantee: Camila Alves de Rezende
• Support Opportunities: Regular Research Grants