Incorporation of indomethacin into a mesoporous silica nanoparticle enhances the anti-inflammatory effect Indomethacin into a mesoporous silica

Author(s):Show less -

Junior, Silvio Almeida ^[1] ; Pereira, Poliana Marques ^[1] ; Totoli, Vanessa de Souza ^[1] ; Neves, Edna Sousa ^[1] ; Monochio, Mayara ^[1] ; Alvarenga, Alef Winter Oliveira ^[1] ; Hori, Juliana Issa ^[2] ; Braz, Wilson Rodrigues ^[3] ; Rocha, Lucas Alonso ^[3] ; Nassar, Eduardo Jose ^[3] ; Aldrovani, Marcela ^[1] ; Furtado, Ricardo Andrade ^[1]

Affiliation

^[1] Univ Franca, Anim Sci Post Grad, Franca, SP - Brazil

^[2] APIS Flora Ind & Comercial LTDA, Ribeirao Preto - Brazil

^[3] Univ Franca, Sci Post Grad, Franca, SP - Brazil

• Document type: Journal article
• Source: European Journal of Pharmaceutical Sciences; v. 157, FEB 1 2021.
• Web of Science Citations: 0

Abstract

Purpose: We evaluated the analgesic, anti-inflammatory and toxicological effects of indomethacin incorporated into mesoporous silica nanoparticles (IND+NP). Methods: Nociception was evaluated by the formalin assay. The anti-inflammatory potential was assessed by cell migration and paw edema assays, modulation of nitric oxide and cytokines (IL-6, IL-10 and TNF-alpha) by macrophages production. Toxicity was evaluated in peritoneal macrophages and by the locomotion assay and assessment of gastric injuries, presence of occult blood and hepatic and renal markers. Results: IND+NP reduced nociception during phases 1 by 53% and 2 by 79% of the formalin assay and the influx of peritoneal cells by 94%, indicating an analgesic and anti-inflammatory effect more efficiently than indomethacin alone. Indomethacin, but not IND+NP, caused macroscopic gastric injuries, the presence of fecal occult blood, and an increase of ALT levels. In the paw edema assay, IND+NP reduced edema by 21%. IND+NP has no effect on the LPS-induced production of nitric oxide, IL-6, IL-10 and TNF-alpha on no cytotoxic concentrations. Conclusions: The incorporation of indomethacin into mesoporous silica nanoparticles effectively increased the activity of the drug observed in the formalin and cell migration assays and prevented the gastric and hepatic damage associated with its use. (AU)

• FAPESP's process: 18/00840-2 - Modulating effect of indomethacin incorporated into silica nanoparticle in the regulation of inflammation mediators
• Grantee: Edna de Sousa Neves
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/10241-6 - Evaluation of anti-inflammatory potential of indomethacin incorpored to mesoporose silica nanoparticle
• Grantee: Ricardo Andrade Furtado
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/22919-7 - Potential of INDOMENTACIN incorporated to the mesoporous silica nanoparticle against inflamation induced by formalin and carragenin
• Grantee: Poliana Marques Pereira
• Support Opportunities: Scholarships in Brazil - Scientific Initiation