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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

ATP-sensitive K+ channels control the spontaneous firing of a glycinergic interneuron in the auditory brainstem

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Author(s):
Strazza, Jr., Paulo S. [1] ; de Siqueira, Daniela V. F. [1] ; Leao, Ricardo M. [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Physiol, Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF PHYSIOLOGY-LONDON; v. 599, n. 5 JAN 2021.
Web of Science Citations: 0
Abstract

Cartwheel neurons from the dorsal cochlear nucleus (DCN) are glycinergic interneurons and the primary source of inhibition on the fusiform neurons, the DCN's principal excitatory neuron. Most cartwheel neurons present spontaneous firing (active neurons), producing a steady inhibitory tone on fusiform neurons. In contrast, a small fraction of these neurons do not fire spontaneously (quiet neurons). Hyperactivity of fusiform neurons is seen in animals with behavioural evidence of tinnitus. Because of its relevance in controlling the excitability of fusiform neurons, we investigated the ion channels responsible for the spontaneous firing of cartwheel neurons in DCN slices from rats. We found that quiet neurons presented an outward conductance not seen in active neurons, which generates a stable resting potential. This current was sensitive to tolbutamide, an ATP-sensitive potassium channel (K-ATP) antagonist. After inhibition with tolbutamide, quiet neurons start to fire spontaneously, while the active neurons were not affected. On the other hand, in active neurons, K-ATP agonist diazoxide activated a conductance similar to quiet neurons' K-ATP conductance and stopped spontaneous firing. According to the effect of K-ATP channels on cartwheel neuron firing, glycinergic neurotransmission in DCN was increased by tolbutamide and decreased by diazoxide. Our results reveal a role of K-ATP channels in controlling the spontaneous firing of neurons not involved in fuel homeostasis. (AU)

FAPESP's process: 19/13458-1 - Changes during the development of ionic currents and neurotransmission of auditory neurons: effects of the acoustic environment
Grantee:Ricardo Mauricio Xavier Leão
Support Opportunities: Regular Research Grants
FAPESP's process: 16/01607-4 - High intensity sound stimulation and synaptic plasticity
Grantee:Ricardo Mauricio Xavier Leão
Support Opportunities: Regular Research Grants