Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Sequential fed batch extractive fermentation for enhanced bioethanol production using recycled Spathaspora passalidarum and mixed sugar composition

Full text
Author(s):
Farias, D. [1] ; Maugeri-Filho, F. [1]
Total Authors: 2
Affiliation:
[1] Univ Campinas UNICAMP, Sch Food Engn, Bioproc & Metab Engn Lab LEMeB, Campinas, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: FUEL; v. 288, MAR 15 2021.
Web of Science Citations: 6
Abstract

The simultaneous ethanol production and removal during sequential cell recycle fed batch fermentation provides a complementary route to produce this biofuel from sugar mixtures, which may greatly improve yields and productivity from lignocellulosic hydrolysates. Spathaspora passalidarum is a wild-type strain able to naturally convert glucose, fructose, xylose and arabinose into ethanol. Therefore, the present work has focused on 2G bioethanol production by S. passalidarum aiming at the consumption of all sugars released after pre-treatment and enzymatic hydrolysis of sugarcane bagasse in a single fermentation step. The fermentation strategy with sequential cell recycle, fed-batch mode and ethanol removal in situ was performed on a hemicellulosic hydrolysate medium supplemented with molasses. This strategy gave improved fermentation performance and enabled the co-fermention of all sugars under microaerobic conditions. The maximum ethanol yield and productivity was 0.482 g.g(-1) and 9.5 g.L-1.h(-1), respectively, showing a process efficiency of 94.3%. The selective ethanol removal enables the operation of the bioreactor at low levels of ethanol (20-30 g.L-1), even with high sugar concentration inputs, accelerating the fermentation performance and avoiding inhibitory effects on yeast metabolism. Applying the cell recycle strategy, S. passalidarum was able to increase its robustness, as shown by a 10-fold increase in ethanol productivity, and it was also able to tolerate a high acetic acid concentration (4.5 g.L-1) during long-term fermentations. These results demonstrate that the bioprocess strategy has a strong potential to improve bioethanol production of rich mixed sugar from lignocellulosic hydrolysates in a single fermentation step. (AU)

FAPESP's process: 15/20630-4 - Biorefinery development integrated to a bioethanol sugar cane plant with zero CO2 emission: routes to convert renewable resources to bio-products and bio-electricity
Grantee:Rubens Maciel Filho
Support Opportunities: Research Projects - Thematic Grants