Hepatotoxicity of the anionic surfactant linear alkylbenzene sulphonate (LAS) in bullfrog tadpoles

Author(s)

Franco-Belussi, Lilian ^{[1, 2, 3]} ; Jones-Costa, Monica ^[1] ; Salla, Raquel Fernanda ^[1] ; Santos Souza, Bruno Fernando ^[1] ; Pinto-Vidal, Felipe Augusto ^{[1, 4]} ; Oliveira, Cristiane Ronchi ^[1] ; Mathias Silva-Zacarin, Elaine Cristina ^[5] ; Abdalla, Fabio Camargo ^[6] ; Silveira Duarte, Iolanda Cristina ^[7] ; De Oliveira, Classius ^[2]

Affiliation

^[1] Univ Fed Sao Carlos UFSCar, Lab Fisiol Conservacao LAFISC, Sao Paulo - Brazil

^[2] Univ Estadual Paulista UNESP, Dept Biol, Sao Jose Do Rio Preto, SP - Brazil

^[3] Univ Fed Mato Grosso do Sul, UFMS, Inst Biociencias INBIO, Lab Patol Expt LAPEx, Ave Costa & Silva S-N, Bairro Univ, Cidade Univ, BR-79070900 Campo Grande, MS - Brazil

^[4] Masaryk Univ, RECETOX, Fac Sci, Kamenice 753-5, Pavil A29, Brno 62500 - Czech Republic

^[5] Univ Fed Sao Carlos, Lab Ecotoxicol & Biomarcadores Anim LEBA, Sao Paulo - Brazil

^[6] Univ Fed Sao Carlos, Lab Biol Estrutural & Func, Sao Paulo - Brazil

^[7] Univ Fed Sao Carlos, Lab Microbiol Ambiental LAMA, Sao Paulo - Brazil

• Document type: Journal article
• Source: Chemosphere; v. 266, MAR 2021.
• Web of Science Citations: 4

Abstract

The liver of anurans play an important role in metabolism, including detoxification, the biotransformation of molecules, and the storage of metabolites. Surfactants are part of domestic and industrial effluents. The effects of linear alkylbenzene sulfonate (LAS) on anuran liver remain unknown, however, some studies have evaluated the effects of LAS on the skin, gills, heart, testes, and liver of fishes. Here, we tested the hypothesis that LAS is hepatotoxic, promoting morphometric alterations in hepatocytes along with inflammation in the tissue, altering hepatic catabolism. We evaluated the effects of a LAS concentration that is considered environmentally safe in Brazilian inland waters on the liver of Lithobates catesbeianus tadpoles, including studies on morphology, morphometry, immunology, and metabolism. LAS exposure promoted enlargement of liver sinusoids and vacuolization of hepatocytes. Exposure to LAS also increased the area of mast cells and melanomacrophages (MMs). Additionally, LAS exposure increased hemosiderin inside MMs, suggesting alterations in the catabolism and storage of iron. Hepatocyte size increased after exposure to LAS, suggesting cytotoxic effects. Integrative analyses (i.e., morphometric, metabolic, and immunological) demonstrated hepatotoxic effects of LAS. These types of studies are key to understanding the negative effects of these substances on tadpole health, as these liver alterations impair anuran homeostasis. (C) 2020 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 08/57712-4 - The National Institute of Comparative Physiological Research
• Grantee: Augusto Shinya Abe
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 14/00946-4 - Internal Pigmentation in Ectotherms: Innate immunity and UV defense
• Grantee: Lilian Franco Belussi
• Support Opportunities: Scholarships in Brazil - Post-Doctoral