Marine associated microbial consortium applied to RBBR textile dye detoxification and decolorization: Combined approach and metatranscriptomic analysis

Author(s)

Licursi Vieira, Gabriela Alves ^[1] ; Cabral, Lucelia ^[1] ; Ramos Otero, Igor Vinicius ^[1] ; Ferro, Milene ^[2] ; de Faria, Adriano Uemura ^[1] ; de Oliveira, Valeria Maia ^[3] ; Bacci, Mauricio ^[2] ; Sette, Lara Duraes ^[1]

Affiliation

^[1] Univ Estadual Paulista, Inst Biociencias, Dept Biol Geral & Aplicada, UNESP, 24A, 1515, BR-13506900 Rio Claro, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Inst Biociencias, Ctr Estudos Insetos Sociais, Rio Claro, SP - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Ctr Pluridisciplinar Pesquisas Quim Biol & Agr, Div Recursos Microbianos, Campinas, SP - Brazil

• Document type: Journal article
• Source: Chemosphere; v. 267, MAR 2021.
• Web of Science Citations: 0

Abstract

The combination of different microorganisms and their metabolisms makes the use of microbial consortia in bioremediation processes a useful approach. In this sense, this study aimed at structuring and selecting a marine microbial consortium for Remazol Brilliant Blue R (RBBR) detoxification and decolorization. Experimental design was applied to improve the culture conditions, and metatranscriptomic analysis to understand the enzymatic pathways. A promising consortium composed of Mucor racemosus CBMAI 847, Marasmiellus sp. CBMAI 1062, Bacillus subtilis CBMAI 707, and Dietzia marls CBMAI 705 was selected. This consortium showed 52% of detoxification and 86% of decolorization in the validation assays after seven days of incubation in the presence of 500 ppm of RBBR. Reduction in RBBR color and toxicity were achieved by biosorption and microbial metabolisms. Metatranscriptomic data indicate that the consortium was able to decolorize and breakdown the RBBR molecule using a coordinated action of oxidases, oxygenases, and hydrolases. Epoxide hydrolases and glyoxalases expression could be associated with the decrease in toxicity. The efficiency of this marine microbial consortium suggests their use in bioremediation processes of textile effluents. (C) 2020 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 13/19486-0 - Marine and Antarctic biotechnology: microbial enzymes and their applications
• Grantee: Lara Durães Sette
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/12098-9 - Marine sulfatases and oxidases for the trimming of biogenic and biobased products (BioTrim)
• Grantee: Lara Durães Sette
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/07957-7 - Marine and Antarctic Mycology: diversity and environmental application
• Grantee: Lara Durães Sette
• Support Opportunities: Regular Research Grants