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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Gene and systemic inflammatory effects and neuroendocrine response in surgical patients anesthetized with desflurane-nitrous oxide or desflurane-nitrous oxide-free: A randomized trial

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Author(s):
Arruda, Nayara M. [1] ; Braz, Leandro G. [1] ; Nogueira, Flavia R. [1] ; Souza, Katina M. [1] ; Aun, Aline G. [1] ; de Carvalho, Lidia R. [2] ; Braz, Jose Reinaldo C. [1] ; Braz, Mariana G. [1]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ UNESP, Botucatu Med Sch, Dept Anesthesiol, GENOTOX Lab, Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Inst Biosci, Dept Biostat, Botucatu, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: International Immunopharmacology; v. 90, JAN 2021.
Web of Science Citations: 0
Abstract

There is growing interest in assessing possible immunotoxicological effects in anesthetized patients. There are controversial findings concerning the effect of nitrous oxide (N2O) anesthetic gas effect on inflammatory response. We tested the hypothesis that N2O associated with desflurane (inhalational anesthetic) was likely to worsen neuro-immune-endocrine effects when compared with desflurane alone in this randomized trial. The primary endpoint of this study was to evaluate the systemic proinflammatory interleukin (IL)-6, and the secondary endpoints included other systemic (IL-1 beta, TNF-alpha, IL-8, IL-10, IL-17A and high-sensitivity C-reactive protein - hs-CRP) and genetic inflammatory markers (NF-kB, IL-6 and COX-2) as well as hormones (adrenocorticotropic hormone, cortisol and prolactin) comparing patients undergoing minor surgery with or without N2O-desflurane. As a second aim, we assessed whether there were changes in the neuro-immune-endocrine profiles within each group. Blood samples were collected before anesthesia, 90 min after anesthesia induction, and the day after surgery. We assessed serum cytokines using a cytometric bead array and hs-CRP by chemiluminescent immunoassay. Expression of three proinflammatory transcripts was assessed by real-time quantitative polymerase chain reaction, and neuroendocrine hormones were detected by chemiluminescent microparticle immunoenzymatic assay. There were no significant between-group differences for any analyzed biomarkers. However, there was a significant increase in: (a) systemic IL-6 and hs-CRP values one day after surgery in both groups and (b) prolactin levels in the intraoperative period compared to baseline and postoperative period levels for both groups. In conclusion, N2O does not impair the inflammatory profile and neuroendocrine response compared to patients who receive only desflurane anesthesia. (AU)

FAPESP's process: 13/16842-0 - Does desflurane anesthesia associated or not with nitrous oxide induce genotoxicity and oxidative stress in patients?
Grantee:Jose Reinaldo Cerqueira Braz
Support Opportunities: Regular Research Grants