Yoneda, Juliana S.
de Araujo, Daniele R.
Liguori, Gabriel R.
Liguori, Tacia T. A.
Moreira, Luiz Felipe P.
Total Authors: 9
 Univ Sao Paulo, Inst Fis, Sao Paulo, SP - Brazil
 Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Synchrotron Light Lab LNLS, POB 6192, BR-13083970 Campinas, SP - Brazil
 Univ Fed ABC, Santo Andre, SP - Brazil
 Univ Politecn Marche, Dipartimento Sci Vita & Ambiente, Ancona - Italy
 Univ Sao Paulo, Hosp Clin HCFMUSP, Fac Med, Inst Coracao InCor, Sao Paulo, SP - Brazil
Total Affiliations: 5
Materials Science & Engineering C-Materials for Biological Applications;
Web of Science Citations:
It is known that guanosine derivatives (G) self-assemble in water forming long, flexible, and interacting aggregates (the so-called G-quadruplexes): by modulating the quadruplex charges, e.g. simply using a mixture of guanosine 5'-monophosphate (GMP) and guanosine (Gua), multi-responsive, self-healing hydrogels can be obtained. In this paper, the potential application of G-hydrogels as drug delivery systems has been assessed. Hydrogels were prepared at different Gua:GMP molar ratios. The photosensitizer Methylene Blue and the proapoptotic protein cytochrome C were used as cargo molecules. Small angle x-ray scattering and atomic force microscopy experiments confirmed the presence of G-quadruplexes disposed in swollen matrices with different mesh-sizes. Rheology measurements showed that the Gua:GMP molar ratio leads to specific drug release mechanisms, as the gel strength is finely tuned by electrostatic repulsion and van der Waals attraction between G-quadruplexes. Noteworthy, the gel cohesion and the drug release were pH responsive. Swelling, self-healing and cell viability features were also investigated: the results qualify the Gua:GMP hydrogel as an excellent biomaterial that can entrap and deliver key biomolecules in a sustained and responsive release manner. (AU)