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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immortalized keratinocytes cells generates an effective model of Epidermal Human Equivalent for irritation and corrosion tests

Full text
Author(s):
Mini, C. A. [1] ; Dreossi, S. A. C. [1] ; Abe, F. R. [1] ; Maria-Engler, S. S. [2] ; Oliveira, D. P. [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto FCFRP USP, Lab Ecotoxicol & Human Toxicol, BR-14040903 Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci FCF USP, Skin Biol & Melanoma Grp, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: TOXICOLOGY IN VITRO; v. 71, MAR 2021.
Web of Science Citations: 0
Abstract

Three-dimensional skin models, also named 3D skin models, human skin equivalents (HSEs), or Human Epidermal Equivalents (HEEs), have been increasingly used for chemical assessments in terms of efficacy and safety. Considering this, we developed an HEE model using immortalized HaCaT cells, aiming to overcome the limitation of primary tissue source. Our 3D model (HaCaT-HEE) exhibited important markers of cell differentiation (CK10, CK14, involucrin, and filaggrin), although the stratum corneum was shown to be modest. Besides, the model showed a good prediction potential considering membrane permeability, sensitivity, specificity, and accuracy in distinguishing irritant and corrosive effects after exposure to selected chemicals recommended by the OECD protocols. We also validated the formazan determination for the MTT method using High-Performance Liquid Chromatography (HPLC). For that, we considered carry over, linearity, reproducibility/robustness, accuracy, precision, selectivity, and matrix effect, according to the Food and Drug Administration (FDA) guideline. Based on our results, we can conclude that our model has an acceptable predictive value for the safety evaluation of compounds after skin exposure, with the great advantage of being constructed using immortalized cells. (AU)

FAPESP's process: 16/15660-4 - Local toxic effects evaluation in epidermis due dyes exposure used in hair dyes
Grantee:Camila Alessandra Mini
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 17/04926-6 - Melanoma and chemoresistance: in vitro and in silico models to exploit therapeutic targets
Grantee:Silvya Stuchi Maria-Engler
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 19/14527-7 - Skin bioprinting as a plataform for alternative tests of efficacy and safety
Grantee:Silvya Stuchi Maria-Engler
Support Opportunities: Regular Research Grants