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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A longitudinal study of convalescent plasma (CCP) donors and correlation of ABO group, initial neutralizing antibodies (nAb), and body mass index (BMI) with nAb and anti-nucleocapsid (NP) SARS-CoV-2 antibody kinetics: Proposals for better quality of CCP collections

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Wendel, Silvano [1] ; Fontao-Wendel, Rita [1] ; Fachini, Roberta [1] ; Candelaria, Gabriela [1] ; Scuracchio, Patricia [1] ; Achkar, Ruth [1] ; Brito, Mayra [1] ; Reis, Luiz Fernando [2] ; Camargo, Anamaria [2] ; Amano, Mariane [2] ; Machado, Rafael [3] ; Araujo, Danielle [3, 4] ; Soares, Camila [3] ; Durigon, Edison [3]
Total Authors: 14
[1] Hosp Sirio Libanes, Blood Bank, Rua Adma Jafet 91, BR-01308050 Sao Paulo - Brazil
[2] Hosp Sirio Libanes, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo - Brazil
[4] Hosp Israelita Albert Einstein, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Transfusion; v. 61, n. 5 FEB 2021.
Web of Science Citations: 4

Introduction Little is known about the neutralizing (nAb) and binding antibody kinetics in COVID-19 convalescent plasma donors, especially during the first 100 days after disease onset. Materials and Methods A cohort of previously RT-PCR positive (detected by nasopharyngeal swab during the acute phase), male convalescent patients, all with mild symptoms, were enrolled in serial blood sample collection for a longitudinal nAb titers and anti-nucleocapsid (NP) antibodies (IgM, IgG and IgA) evaluation. NAbs were detected by a cytopathic effect-based virus neutralization test (CPE-based VNT), carried out with SARS-CoV-2 (GenBank: MT350282). Results A total of 78 male volunteers provided 316 samples, spanning a total of 4820 days of study. Although only 25% of donors kept nAb titers >= 160 within 100 days after the onset of disease, there was >75% probability of sustaining nAb titers >= 160 in volunteers whose initial nAb titer was >= 1280, weight >= 90 kg or obese, according to their body mass index (BMI), as evidenced by Kaplan-Meier analysis and Cox hazard regression (all p < .02). There was no correlation between the ABO group, ABO antibody titers and persistent high nAb titers. High IgG anti-NP (S/CO >= 5.0) is a good surrogate for detecting nAb >= 160, defined by the ROC curve (sensitivity = 90.5%; CI95%: 84.5%-94.7%). Conclusion Selection of CCP donors for multiple collections based on initial high nAb titers (>= 1280) or BMI >= 30 kg/m(2) provides a simple strategy to achieve higher quality in CCP programs. High IgG anti-NP levels can also be used as surrogate markers for high nAb screening. (AU)

FAPESP's process: 20/06409-1 - Evaluation of humoral immune response and inflammatory response in patients with confirmed diagnosis of COVID-19 at Hospital Sírio Libanês and correlation with disease severity
Grantee:Edison Luiz Durigon
Support type: Regular Research Grants
FAPESP's process: 16/20045-7 - Antigen discovery and development of serological diagnostic methods and vaccine approaches against the Zika Virus (ZIKV)
Grantee:Luis Carlos de Souza Ferreira
Support type: Research Projects - Thematic Grants
FAPESP's process: 17/24769-2 - Zika virus in postpartum women and newborns: seroepidemiology and molecular characterization
Grantee:Rafael Rahal Guaragna Machado
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 18/23680-0 - Standardization of Sherlock technique (Specific High-Sensitivity Enzymatic Reporter Unlocking) using the Cas13a protein for the diagnosis of respiratory syncytial virus in pediatric patients
Grantee:Camila Pereira Soares
Support type: Scholarships in Brazil - Master