Production of beta-glucan exopolysaccharide lasiodiplodan by Lasiodiplodia theobromae CCT 3966 from corn bran acid hydrolysate

Author(s)

Philippini, Rafael Rodrigues ^[1] ; Martiniano, Sabrina Evelin ^[1] ; Franco Marcelino, Paulo Ricardo ^[1] ; Chandel, Anuj Kumar ^[1] ; dos Santos, Julio Cesar ^[1] ; Da Silva, Silvio Silverio ^[1]

Affiliation

^[1] Univ Sao Paulo, Engn Sch Lorena, Dept Biotechnol, Estr Municipal Campinho S-N 12, BR-602810 Lorena, SP - Brazil

• Document type: Journal article
• Source: Applied Microbiology and Biotechnology; v. 105, n. 6 FEB 2021.
• Web of Science Citations: 0

Abstract

The potential utilization of corn bran acid hydrolysate (CBAH) was evaluated as an inexpensive feedstock for the production of a rich carbohydrate and protein medium for lasiodiplodan (LAS) production using the filamentous fungus Lasiodiplodia theobromae CCT 3966. Experiments were performed according to a 2(2) CCRD experimental design aiming to evaluate the influence of agitation speed (rpm) and temperature (degrees C) over the production of total cell biomass (TCB) and LAS concentration released to the medium (LAS-M), adhered to biomass (LAS-C), and total (LAS-T). Under the selected conditions (temperature of 28 degrees C and agitation of 200 rpm), 8.73 g center dot L-1 of LAS-T and 4.47 g center dot L-1 of TCB were obtained. Recovery of LAS-C with hot water was shown as an alternative to increase the production concentration, although it might require further purification steps. CBAH potential for substitution of synthetic media was demonstrated, indicating that it is an adequate raw material containing all necessary nutrients for LAS production. (AU)

• FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
• Grantee: Roberto de Campos Giordano
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants