Functionalized Cellulose Nanocrystals for Cellular Labeling and Bioimaging

Author(s):Show less -

Raja, Sebastian ^{[1, 2]} ; Hamouda, I, Ahmed E. ; de Toledo, Marcelo A. S. ^[3] ; Hu, Chaolei ^[4] ; Bernardo, Marcela P. ^{[1, 3]} ; Schalla, Carmen ^[3] ; Leite, Liliane S. F. ^[1] ; Buhl, Eva Miriam ^[5] ; Dreschers, Stephan ^[6] ; Pich, Andrij ^[4] ; Zenke, Martin ^[3] ; Mattoso, Luiz H. C. ^[1] ; Sechi, Antonio ^[3]

Affiliation

^[1] EMBRAPA Instrumentacao, Natl Nanotechnol Lab Agribusiness LNNA, BR-13560970 Sao Carlos, SP - Brazil

^[2] Rhein Westfal TH Aachen, Inst Biomed Engn, Dept Cell Biol, D-52074 Aachen - Germany

^[3] Hamouda, Ahmed E., I, Rhein Westfal TH Aachen, Inst Biomed Engn, Dept Cell Biol, D-52074 Aachen - Germany

^[4] Rhein Westfal TH Aachen, DWI Leibniz Inst Interact Mat, D-52074 Aachen - Germany

^[5] Rhein Westfal TH Aachen, Inst Pathol, Electron Microscopy Facil, D-52074 Aachen - Germany

^[6] Rhein Westfal TH Aachen, Klin Kinder & Jugendmed, D-52074 Aachen - Germany

• Document type: Journal article
• Source: Biomacromolecules; v. 22, n. 2, p. 454-466, FEB 2021.
• Web of Science Citations: 0

Abstract

Cellulose nanocrystals (CNCs) are unique and promising natural nanomaterials that can be extracted from native cellulose fibers by acid hydrolysis. In this study, we developed chemically modified CNC derivatives by covalent tethering of PEGylated biotin and perylenediimide (PDI)-based near-infrared organic dye and evaluated their suitability for labeling and imaging of different cell lines including J774A.1 macrophages, NIH-3T3 fibroblasts, HeLa adenocarcinoma cells, and primary murine dendritic cells. PDI-labeled CNCs showed a superior photostability compared to similar commercially available dyes under long periods of constant and high-intensity illumination. All CNC derivatives displayed excellent cytocompatibility toward all cell types and efficiently labeled cells in a dose-dependent manner. Moreover, CNCs were effectively internalized and localized in the cytoplasm around perinuclear areas. Thus, our findings demonstrate the suitability of these new CNC derivatives for labeling, imaging, and long-time tracking of a variety of cell lines and primary cells. (AU)

• FAPESP's process: 15/00094-0 - Chemical modifications of cellulose nanowhiskers
• Grantee: Raja Sebastian
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 17/22017-3 - Near - infrared (NIR) cellulose nanocrystals (NIR - CNC) for cellular bioimaging
• Grantee: Raja Sebastian
• Support type: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 16/03080-3 - Preparation and characterization of bionanocomposites based on proteins and cellulose nanocrystals by continuous casting
• Grantee: Liliane Samara Ferreira Leite
• Support type: Scholarships in Brazil - Doctorate