Hybridisation experiments between freshwater prawns Macrobrachium amazonicum and M. pantanalense (Decapoda: Palaemonidae), and the effects of geographical isolation

Author(s)

Nogueira, Caio Santos ^[1] ; Pantaleao, Joao Alberto Farinelli ^[2] ; Costa, Rogerio Caetano ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Dept Biol Sci, Fac Sci, Lab Biol Marine & Freshwater Shrimps LABCAM, Ave Engenheiro Luiz Edmundo Carrijo Coube 14-01, BR-17033360 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto FFCLR, Dept Biol, Lab Bioecol & Systemat Crustacean LBSC, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: MARINE AND FRESHWATER RESEARCH; v. 72, n. 4, p. 520-525, 2021.
• Web of Science Citations: 0

Abstract

The freshwater prawnsMacrobrachium amazonicumandM. pantanalenseare phylogenetically closely related. The present study analysed the occurrence of copulation between these two species. Specimens ofM. amazonicumwere collected from the Tiete River in Cambaratiba (SP), whereasM. pantanalensespecimens were collected from the Lagoa Baiazinha, in Miranda (MS). Six different experimental groups were used, MA male x MP female, MA female x MP male (interspecifc), MA male x MA female, MP male x MP female (intraspecific), MA female and MP female (individualised; MA = M. amazonicumand MP = M. pantanalense). The incubation times of the ovigerous females were monitored in all experiments. Some females from all experimental groups were randomly selected and had their oocytes photographed to verify the occurrence of embryonic development. No larvae hatched in the interspecific experimental groups, whereas, in intraspecific groups, almost all experiments (95%) presented larval hatching. The incubation period differed significantly (P < 0.001) between the intraspecific groups and all the other experimental groups, but not between each other (P > 0.05). The interspecific and individualised groups showed no significant (P > 0.05) difference between them. In addition, oocytes from the interspecific experimental groups did not present characteristics of embryonic development. Our results suggest thatM. pantanalenseandM. amazonicumpopulations do not copulate, which corroborates the proposal of recent speciation. (AU)

• FAPESP's process: 19/00661-3 - A comparative interdisciplinary analysis between the prawns Macrobrachium amazonicum (Heller, 1862) and Macrobrachium pantanalense (dos Santos, Hayd & anger, 2013) support the interspecific differentiation?
• Grantee: Caio dos Santos Nogueira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/13685-5 - Integrative analysis of the Brazilian fauna of decapod crustaceans: taxonomy, phylogenetic systematics, spermiotaxonomy, morphology of post-embryonic development, ecology and conservation
• Grantee: Fernando Luis Medina Mantelatto
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants