Melanin pigmented gingival tissue impairs red-light lateral scattering for antimicrobial photodynamic therapy

Author(s)

Fruet-Arruda, Regina T. ^{[1, 2]} ; Anselmo, Giuliana Giovinazzo ^{[1, 2]} ; Tortamano, Ana Carolina A. C. ^{[1, 2]} ; Rossi, Adriana L. ^{[1, 2]} ; Biffi, Maria Beatriz ^{[1, 2]} ; Marco, Rodrigo Labat ^[2] ; da Silva, Daniela de F. T. ^[2] ; Kato, Ilka T. ^[3] ; Nunez, Silvia C. ^[4] ; Prates, Renato A. ^{[1, 2]}

Affiliation

^[1] Nove de Julho Univ UNINOVE, Sch Dent, Sao Paulo - Brazil

^[2] Nove de Julho Univ UNINOVE, Biophoton Appl Hlth Sci Post Grad Program, Sao Paulo - Brazil

^[3] ABC Fed Univ UFABC, Dept Biomed Engn, Sao Bernardo Do Campo, SP - Brazil

^[4] Univ Brasil, Dept Bioengn, Sao Paulo - Brazil

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 33, MAR 2021.
• Web of Science Citations: 0

Abstract

Gingival melanin pigmentation is present in many African and Oriental descendant people and its occurrence in patients may interfere with the absorption and scattering of therapeutic doses of light. Antimicrobial photody-namic therapy (aPDT) is used as an adjunctive treatment for periodontitis and light irradiation may be impaired by tissue size and its melanin content. The aim of this clinical study was to measure the red-light attenuation in gingival tissue naturally pigmented with melanin. Ten patients with melanized gingival tissue were selected and irradiated by 100 mW red laser. The patients were photographed in frontal and incisal regions with a T2i camera (Canon, Japan) with 100 mm macro lens, 35 mm focal length, aperture f22, 1/100 shutter speed and ISO 200. Three randomly selected sites of each patient were used for evaluations and the irradiation values were assessed in the IMAGEJ software (NIH, Wayne Rasband, USA). Intensity in pixels was quantified in relation to the distance from the light incident point. Data were normalized and the results were presented as relative light intensity as a function of distance. The results demonstrated that red laser light is exponentially attenuated as a function of lateral distance and loses approximately 50 % of its intensity by 2.23 mm. On the other hand, the light travels 3 mm in depth to decay 50 %. In conclusion, our data suggest that melanin presence decreases optical pathway and irradiation protocols for antimicrobial photodynamic therapy in gingival tissue should consider light attenuation and depth of periodontal pockets so that efficient illumination of the target tissue occurs. Periodontal pockets bigger than 6 mm should be irradiated with more than one point. (AU)

• FAPESP's process: 16/10269-5 - Evaluation of Parameters of Tissue Dosimetry in Photodynamic Therapy for the Treatment of Periodontal Disease - clinical study
• Grantee: Renato Araujo Prates
• Support Opportunities: Regular Research Grants