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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The In Vitro Effect of Ozone Therapy Against Equine Pythium insidiosum

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Author(s):
Ferreira, Jair Camargo [1] ; Pires, Regina Helena [2] ; da Costa, Guilherme Barbosa [1] ; Carrijo, Bruna Nonato [1] ; Guiotto, Francine Gabriela [1] ; Rodrigues, Viviani Silva [1]
Total Authors: 6
Affiliation:
[1] Univ Franca, Postgrad Program Anim Sci, Sao Paulo - Brazil
[2] Univ Franca, Postgrad Program Hlth Promot, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Equine Veterinary Science; v. 98, MAR 2021.
Web of Science Citations: 0
Abstract

The goal of the present study was to characterize the antimicrobial action of different ozone (O-3) presentations against Pythium insidiosum isolated from horses. In experiment 1, P. insidiosum was treated with ozonated distilled water, ozonated sunflower oils with distinct peroxide indexes or O-3 gas (72 mu g O-3 mL(-1)). In experiment 2, samples were exposed one or three times to oxygen (O-2) or O-3 gas (72 mu g O-3 mL(-1); 30 min/ day). In experiment 3, P. insidiosum was treated with different concentrations of O-3 gas (\& Oslash;, 32, 52, or 72 mu g O-3 mL(-1)) for three days (30 min/day). In experiment 4, samples were exposed to O-2 or O-3 gas (72 mu g O-3 mL(-1)) for 05,15, or 30 minutes during three days. Posteriorly, all samples were cultured for two weeks, and the pathogen growth area was measured until D14. Samples with absence of growth on D14 were recultured to assess the germicidal or germistatic action of the treatment. In experiment 1, only ozonized sunflower oil with a high peroxide index had germicidal action against the pathogen. In experiment 2, samples exposed three times to O-3 gas were inactivated. In experiment 3, the O-3 therapy had germicidal action against P. insidiosum independently of the gas concentration (P > .1). In experiment 4, O-3 treatments >15 minutes suppressed the pathogen development, whereas samples exposed to O-3 gas for 5 minutes had progressive growth (P < .01). In conclusion, ozonated sunflower oil with a high peroxide index and multiple exposures to O-3 gas mixtures were able to inactivate P. insidiosum isolated from horses. (C) 2020 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 19/13812-0 - Different types of ozone administration in the inactivation of Pythium insidiosum - in vitro study
Grantee:Francine Gabriela Guiotto
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 19/15532-4 - Inactivation of Pythium insidiosum by ozone gas: effect of dose and time of exposure
Grantee:Viviani Silva Rodrigues
Support Opportunities: Scholarships in Brazil - Scientific Initiation