2-acetylphenothiazine protects L929 fibroblasts against UVB-induced oxidative damage

Author(s)

Alves da Silva, Bruna Terra ^[1] ; Peloi, Karen Elaine ^[1] ; Ximenes, Valdecir Farias ^[2] ; Nakamura, Celso Vataru ^{[1, 3]} ; Silva Lautenschlager, Sueli de Oliveira ^{[1, 3]}

Affiliation

^[1] Univ Estadual Maringa, Postgrad Program Pharmaceut Sci, Maringa, Parana - Brazil

^[2] Sao Paulo State Univ, UNESP, Dept Chem, Fac Sci, BR-17033360 Bauru, SP - Brazil

^[3] Maringa State Univ UEM, Dept Basic Hlth Sci, BR-87020900 Maringa, Parana - Brazil

• Document type: Journal article
• Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 216, MAR 2021.
• Web of Science Citations: 0

Abstract

Ultraviolet B (UVB) light corresponds to 5% of ultraviolet radiation. It is more genotoxic and mutagenic than UVA and causes direct and indirect cellular damage through the generation of reactive oxygen species (ROS). Even after radiation, ROS generation may continue through activation of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) enzyme. Long-term exposure can progress to premature skin aging and photocarcinogenesis. To prevent damage that is caused by UVB radiation, several studies have focused on the topical administration of compounds that have antioxidant properties. 2-Acetylphenothiazine (ML171) is a potent and selective inhibitor of NOX1. The present study investigated the antioxidant potential and photo-protective ability of ML171 in UVB-irradiated L929 fibroblasts. ML171 had considerable antioxidant activity in both the DPPH? and xanthine/luminol/xanthine oxidase assays. ML171 did not induce cytotoxicity in L929 fibroblasts and increased the viability of UVB-irradiated cells. ML171 also inhibited ROS production, the enzy-matic activity of NOX, depolarization of the mitochondrial membrane, and DNA damage. Additionally, ML171 protected cell membrane integrity and induced fibroblast migration. These results suggest that the incorporation of ML171 in topical administration systems may be a promising strategy to mitigate UVB-induced oxidative damage in L929 fibroblasts. (AU)

• FAPESP's process: 14/50926-0 - INCT 2014: biodiversity and natural products
• Grantee: Vanderlan da Silva Bolzani
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 19/18445-5 - Synthesis, studies and applications of fluorescent and circular dichroic probes for characterization of interactions with proteins, DNA and determination of enzymatic activity
• Grantee: Valdecir Farias Ximenes
• Support Opportunities: Regular Research Grants